Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View

The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by ch...

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Main Authors: Petar Eftimov, Agnieszka Olżyńska, Adéla Melcrová, Georgi As. Georgiev, Philippe Daull, Jean-Sebastien Garrigue, Lukasz Cwiklik
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/21/24/9490
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spelling doaj-c1d60a6288994fc9909a514d13df28702020-12-15T00:01:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-01219490949010.3390/ijms21249490Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical ViewPetar Eftimov0Agnieszka Olżyńska1Adéla Melcrová2Georgi As. Georgiev3Philippe Daull4Jean-Sebastien Garrigue5Lukasz Cwiklik6Department of Cytology and Embryology, Faculty of Biology, University of Sofia, 1504 Sofia, BulgariaJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, Dolejškova 3, 18223 Prague, Czech RepublicJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, Dolejškova 3, 18223 Prague, Czech RepubliciBB—Institute for Bioengineering and Biosciences, Complexo Interdisciplinar, IST, Universidade de Lisboa, 1649-004 Lisbon, PortugalSANTEN SAS, Novagali Innovation Center, 1, rue Pierre Fontaine, Bâtiment Genavenir IV, CEDEX, F-91458 Evry, FranceSANTEN SAS, Novagali Innovation Center, 1, rue Pierre Fontaine, Bâtiment Genavenir IV, CEDEX, F-91458 Evry, FranceJ. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, Dolejškova 3, 18223 Prague, Czech RepublicThe tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface.https://www.mdpi.com/1422-0067/21/24/9490tear filmmeibumtear film lipid layerdilatation rheologyfluorescence microscopymolecular dynamics
collection DOAJ
language English
format Article
sources DOAJ
author Petar Eftimov
Agnieszka Olżyńska
Adéla Melcrová
Georgi As. Georgiev
Philippe Daull
Jean-Sebastien Garrigue
Lukasz Cwiklik
spellingShingle Petar Eftimov
Agnieszka Olżyńska
Adéla Melcrová
Georgi As. Georgiev
Philippe Daull
Jean-Sebastien Garrigue
Lukasz Cwiklik
Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
International Journal of Molecular Sciences
tear film
meibum
tear film lipid layer
dilatation rheology
fluorescence microscopy
molecular dynamics
author_facet Petar Eftimov
Agnieszka Olżyńska
Adéla Melcrová
Georgi As. Georgiev
Philippe Daull
Jean-Sebastien Garrigue
Lukasz Cwiklik
author_sort Petar Eftimov
title Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_short Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_full Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_fullStr Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_full_unstemmed Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View
title_sort improving stability of tear film lipid layer via concerted action of two drug molecules: a biophysical view
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2020-12-01
description The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops–tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear–air interface.
topic tear film
meibum
tear film lipid layer
dilatation rheology
fluorescence microscopy
molecular dynamics
url https://www.mdpi.com/1422-0067/21/24/9490
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