Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In th...
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doaj-c1425c160fc8476aada2328f1584143f2020-11-25T03:31:19ZengMDPI AGNutrients2072-66432020-09-01122706270610.3390/nu12092706Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 CellsAkshay Bisht0Martin Dickens1Kay Rutherfurd-Markwick2Rohith Thota3Anthony N. Mutukumira4Harjinder Singh5School of Food and Advanced Technology, College of Science, Massey University, Auckland 0745, New ZealandSchool of Health Sciences, College of Health, Massey University, Auckland 0745, New ZealandSchool of Health Sciences, College of Health, Massey University, Auckland 0745, New ZealandRiddet Research Institute, Massey University, Palmerston North 4442, New ZealandSchool of Food and Advanced Technology, College of Science, Massey University, Auckland 0745, New ZealandRiddet Research Institute, Massey University, Palmerston North 4442, New ZealandThe anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.https://www.mdpi.com/2072-6643/12/9/2706curcuminchlorogenic acidbioactive combinationinflammationNF-κB pathway |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Akshay Bisht Martin Dickens Kay Rutherfurd-Markwick Rohith Thota Anthony N. Mutukumira Harjinder Singh |
spellingShingle |
Akshay Bisht Martin Dickens Kay Rutherfurd-Markwick Rohith Thota Anthony N. Mutukumira Harjinder Singh Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells Nutrients curcumin chlorogenic acid bioactive combination inflammation NF-κB pathway |
author_facet |
Akshay Bisht Martin Dickens Kay Rutherfurd-Markwick Rohith Thota Anthony N. Mutukumira Harjinder Singh |
author_sort |
Akshay Bisht |
title |
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells |
title_short |
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells |
title_full |
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells |
title_fullStr |
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells |
title_full_unstemmed |
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells |
title_sort |
chlorogenic acid potentiates the anti-inflammatory activity of curcumin in lps-stimulated thp-1 cells |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-09-01 |
description |
The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells. |
topic |
curcumin chlorogenic acid bioactive combination inflammation NF-κB pathway |
url |
https://www.mdpi.com/2072-6643/12/9/2706 |
work_keys_str_mv |
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