Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells

The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In th...

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Main Authors: Akshay Bisht, Martin Dickens, Kay Rutherfurd-Markwick, Rohith Thota, Anthony N. Mutukumira, Harjinder Singh
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Nutrients
Subjects:
Online Access:https://www.mdpi.com/2072-6643/12/9/2706
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spelling doaj-c1425c160fc8476aada2328f1584143f2020-11-25T03:31:19ZengMDPI AGNutrients2072-66432020-09-01122706270610.3390/nu12092706Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 CellsAkshay Bisht0Martin Dickens1Kay Rutherfurd-Markwick2Rohith Thota3Anthony N. Mutukumira4Harjinder Singh5School of Food and Advanced Technology, College of Science, Massey University, Auckland 0745, New ZealandSchool of Health Sciences, College of Health, Massey University, Auckland 0745, New ZealandSchool of Health Sciences, College of Health, Massey University, Auckland 0745, New ZealandRiddet Research Institute, Massey University, Palmerston North 4442, New ZealandSchool of Food and Advanced Technology, College of Science, Massey University, Auckland 0745, New ZealandRiddet Research Institute, Massey University, Palmerston North 4442, New ZealandThe anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.https://www.mdpi.com/2072-6643/12/9/2706curcuminchlorogenic acidbioactive combinationinflammationNF-κB pathway
collection DOAJ
language English
format Article
sources DOAJ
author Akshay Bisht
Martin Dickens
Kay Rutherfurd-Markwick
Rohith Thota
Anthony N. Mutukumira
Harjinder Singh
spellingShingle Akshay Bisht
Martin Dickens
Kay Rutherfurd-Markwick
Rohith Thota
Anthony N. Mutukumira
Harjinder Singh
Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
Nutrients
curcumin
chlorogenic acid
bioactive combination
inflammation
NF-κB pathway
author_facet Akshay Bisht
Martin Dickens
Kay Rutherfurd-Markwick
Rohith Thota
Anthony N. Mutukumira
Harjinder Singh
author_sort Akshay Bisht
title Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
title_short Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
title_full Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
title_fullStr Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
title_full_unstemmed Chlorogenic Acid Potentiates the Anti-Inflammatory Activity of Curcumin in LPS-Stimulated THP-1 Cells
title_sort chlorogenic acid potentiates the anti-inflammatory activity of curcumin in lps-stimulated thp-1 cells
publisher MDPI AG
series Nutrients
issn 2072-6643
publishDate 2020-09-01
description The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-α production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-α production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-α production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-κB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-α (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-κB (~78%), IκB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.
topic curcumin
chlorogenic acid
bioactive combination
inflammation
NF-κB pathway
url https://www.mdpi.com/2072-6643/12/9/2706
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