Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria

Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria

Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylob...

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Main Authors: Ndudim I. Ogo, Emmanuel Onovoh, Oluyinka O. Okubanjo, Ruth C. Galindo, Jose-Manuel P. de la Lastra, Jose de la Fuente
Format: Article
Language:English
Published: AOSIS 2012-02-01
Series:Onderstepoort Journal of Veterinary Research
Subjects:
Cordylobia anthropophaga
dog
molecular identification
myiasis
Nigeria
Online Access:https://ojvr.org/index.php/ojvr/article/view/349
id doaj-c0fe2ddabce849d2a5c49aeb6a72abbc
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spelling doaj-c0fe2ddabce849d2a5c49aeb6a72abbc2020-11-24T23:23:10ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352012-02-01791e1e410.4102/ojvr.v79i1.349302Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, NigeriaNdudim I. Ogo0Emmanuel Onovoh1Oluyinka O. Okubanjo2Ruth C. Galindo3Jose-Manuel P. de la Lastra4Jose de la Fuente5National Veterinary Research Institute, VomNational Veterinary Research Institute, VomDepartment of Veterinary Parasitology, Ahmadu Bello UniversityInstituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad RealInstituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad RealInstituto de Investigacion en Recursos Cinegiticos, IREC (CSIC-UCLM-JCCM), Ciudad RealMyiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.https://ojvr.org/index.php/ojvr/article/view/349Cordylobia anthropophagadogmolecular identificationmyiasisNigeria
collection DOAJ
language English
format Article
sources DOAJ
author Ndudim I. Ogo
Emmanuel Onovoh
Oluyinka O. Okubanjo
Ruth C. Galindo
Jose-Manuel P. de la Lastra
Jose de la Fuente
spellingShingle Ndudim I. Ogo
Emmanuel Onovoh
Oluyinka O. Okubanjo
Ruth C. Galindo
Jose-Manuel P. de la Lastra
Jose de la Fuente
Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
Onderstepoort Journal of Veterinary Research
Cordylobia anthropophaga
dog
molecular identification
myiasis
Nigeria
author_facet Ndudim I. Ogo
Emmanuel Onovoh
Oluyinka O. Okubanjo
Ruth C. Galindo
Jose-Manuel P. de la Lastra
Jose de la Fuente
author_sort Ndudim I. Ogo
title Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
title_short Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
title_full Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
title_fullStr Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
title_full_unstemmed Molecular identification of <i>Cordylobia anthropophaga</i > Blanchard (Diptera: Calliphoridae) larvae collected from dogs (<i>Canis familiaris</i>) in Jos South, Plateau State, Nigeria
title_sort molecular identification of <i>cordylobia anthropophaga</i > blanchard (diptera: calliphoridae) larvae collected from dogs (<i>canis familiaris</i>) in jos south, plateau state, nigeria
publisher AOSIS
series Onderstepoort Journal of Veterinary Research
issn 0030-2465
2219-0635
publishDate 2012-02-01
description Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.
topic Cordylobia anthropophaga
dog
molecular identification
myiasis
Nigeria
url https://ojvr.org/index.php/ojvr/article/view/349
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