Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment

Abstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of...

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Main Authors: Sachi Sugita, Masanori Yamato, Toshimitsu Hatabu, Yosky Kataoka
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-88414-1
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spelling doaj-c0ca0590a75d41e6951ed60788a9057f2021-05-23T11:34:12ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111110.1038/s41598-021-88414-1Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironmentSachi Sugita0Masanori Yamato1Toshimitsu Hatabu2Yosky Kataoka3Laboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama UniversityLaboratory for Cellular Function Imaging, RIKEN Center for Biosystems Dynamics ResearchLaboratory of Animal Physiology, Graduate School of Environmental and Life Science, Okayama UniversityLaboratory for Cellular Function Imaging, RIKEN Center for Biosystems Dynamics ResearchAbstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of cancer, and the maximum standardized uptake value (SUVmax) has been correlated with poor prognosis. However, the phenotype of cells that show high 18F-FDG accumulation in tumors remains unknown. Here, we combined quantitative micro-autoradiography with fluorescence immunohistochemistry to simultaneously visualize 18F-FDG distribution, the expression of multiple proteins, and hypoxic regions in the cancer microenvironment of a human A431 xenograft tumor in C.B-17/Icr-scid/scid mice. We found that the highest 18F-FDG accumulation was in cancer-derived cells undergoing epithelial-mesenchymal transition (EMT) in hypoxic regions, implicating these regions as a major contributor to increased glucose metabolism, as measured by 18F-FDG-PET.https://doi.org/10.1038/s41598-021-88414-1
collection DOAJ
language English
format Article
sources DOAJ
author Sachi Sugita
Masanori Yamato
Toshimitsu Hatabu
Yosky Kataoka
spellingShingle Sachi Sugita
Masanori Yamato
Toshimitsu Hatabu
Yosky Kataoka
Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
Scientific Reports
author_facet Sachi Sugita
Masanori Yamato
Toshimitsu Hatabu
Yosky Kataoka
author_sort Sachi Sugita
title Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_short Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_full Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_fullStr Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_full_unstemmed Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_sort involvement of cancer-derived emt cells in the accumulation of 18f-fluorodeoxyglucose in the hypoxic cancer microenvironment
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-05-01
description Abstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of cancer, and the maximum standardized uptake value (SUVmax) has been correlated with poor prognosis. However, the phenotype of cells that show high 18F-FDG accumulation in tumors remains unknown. Here, we combined quantitative micro-autoradiography with fluorescence immunohistochemistry to simultaneously visualize 18F-FDG distribution, the expression of multiple proteins, and hypoxic regions in the cancer microenvironment of a human A431 xenograft tumor in C.B-17/Icr-scid/scid mice. We found that the highest 18F-FDG accumulation was in cancer-derived cells undergoing epithelial-mesenchymal transition (EMT) in hypoxic regions, implicating these regions as a major contributor to increased glucose metabolism, as measured by 18F-FDG-PET.
url https://doi.org/10.1038/s41598-021-88414-1
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