Accessible morphohistochemical labs-on-a-chip based on different counting chambers' grids: microfluidic morphodynamical workstations

• Main Authors: Gradov O.V., Notchenko A.V.
• Format: Article
• Language: Russian
• Published: Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy 2012-01-01
• Series: Морфологія
• Subjects: lab-on-a-chip; haemocytometer; counting chambers; hematimeter; mathematical morphology; computer morphometry; 3D luminance surface; digital morphogenesis
• Online Access: http://www.morphology.dp.ua/_pub/MORPHO-2012-06-01/12govmrs.pdf

An accessible design of autonomous labs on the chip which do not require a special reader is developed. The proposed device uses hemocytometric counting chambers for determinati on of concentration of cells, isolated by a special device for cell sedimentation. A system of automated Rf-identification of chambers in the long-term storage is proposed which provides new morphometric data at various stages of cultivation or biomonitoring. A ne w diffraction method of calcu-lation and fingerprinting of cellular structures in varying environmental conditions is described. Experimental data on the pilot testing of Fuchs-Rosenthal, Buerker, Neubauer, Makler and Thoma chambers fo r the described technique is given. The applicability of these devices for coherent-d ensitometric indication either of concen tration dynamics in cell culture or suspen -sion or morphogenetic processes in them for laminar conditions is shown. A self-learning program for cellular pattern recog-nition and relational cytophotometry based on lab on the chip w ith an object-micrometric calib ration relative to the counting net is demonstrated. A hybrid in vitro / in silico met hod of morphogenesis monitoring in cell cultures is introduced.