| Summary: | PGRMC1 is a multifunctional protein that is found in multiple subcellular compartments, suggesting a
specific function at each site. Among the several subcellular sites of expression, PGRMC1 was found in
the nucleolus of human cells (Ahmad et al. 2009) and bovine zygotes (Luciano et al. 2010). However, the
role at this site is not clear. Therefore, the aim of this study is to assess whether PGRMC1 modulates
nucleolar function. Immunofluorescence experiments confirmed nucleolar localization in rat
spontaneously immortalized granulosa cells, bovine granulosa cells (bGC) and bovine oocytes.
Moreover, in bGC PGRMC1 co-localizes with nucleolin, a well-known nucleolar marker exerting
important functions within the nucleolus. Additionally, siRNA mediated gene knockdown experiments
showed that when PGRMC1 expression is silenced, nucleolin localization shifts from the nucleolus to the
nucleoplasm, suggesting a PGRMC1/nucleolin functional association. However, in situ proximity ligation
assay did not detect a direct interaction between these two proteins, suggesting the involvement of
additional molecules that could mediate PGRMC1/nucleolin interaction.
In conclusion, these studies suggest a function for PGRMC1 in nucleolar activity and set the stage for
further investigations aimed at dissecting PGRMC1’s molecular mechanisms of action in the nuclear
compartment.
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