Novel organic solvent-tolerant esterase isolated by metagenomics: insights into the lipase/esterase classification Nueva esterasa tolerante a los solventes orgánicos aislada por metagenómica: ideas sobre la clasificación de las esterasas/lipasas
In order to isolate novel organic solvent-tolerant (OST) lipases, a metagenomic library was built using DNA derived from a temperate forest soil sample. A two-step activity-based screening allowed the isolation of a lipolytic clone active in the presence of organic solvents. Sequencing of the plasmi...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier España
2013-03-01
|
Series: | Revista Argentina de Microbiología |
Subjects: | |
Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412013000100002 |