The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

The anti-angiogenic and neurogenic pigment epithelium-derived factor (PEDF) demonstrated a potency to control choroidal neovascularization in age-related macular degeneration (AMD) patients. The goal of the present study was the development of an efficient and safe technique to integrate, ex vivo, t...

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Bibliographic Details
Main Authors: Marie Pastor, Sandra Johnen, Nina Harmening, Mickäel Quiviger, Julie Pailloux, Martina Kropp, Peter Walter, Zoltán Ivics, Zsuzsanna Izsvák, Gabriele Thumann, Daniel Scherman, Corinne Marie
Format: Article
Language:English
Published: Elsevier 2018-06-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
age-related macular degeneration
electroporation
RPE cells
antibiotic-free pFAR4 vector
Sleeping Beauty transposon
ocular gene therapy
transfection
PEDF
VEGF
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117303189

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http://www.sciencedirect.com/science/article/pii/S2162253117303189

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