Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling

Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling

Abstract Background CRISPR/CAS9 (epi)genome editing revolutionized the field of gene and cell therapy. Our previous study demonstrated that a rapid and robust reactivation of the HIV latent reservoir by a catalytically-deficient Cas9 (dCas9)-synergistic activation mediator (SAM) via HIV long termina...

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Bibliographic Details
Main Authors: Yonggang Zhang, Gustavo Arango, Fang Li, Xiao Xiao, Raj Putatunda, Jun Yu, Xiao-Feng Yang, Hong Wang, Layne T. Watson, Liqing Zhang, Wenhui Hu
Format: Article
Language:English
Published: BMC 2018-09-01
Series:BMC Medical Genomics
Subjects:
Genome editing
CRISPR
Off-target
RNA sequencing
Transcriptome
HIV
Online Access:http://link.springer.com/article/10.1186/s12920-018-0394-2

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