The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase

The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase

The RNA-guided Cas9 nuclease, from the type II prokaryotic clustered regularly interspersed short palindromic repeats (CRISPR) adaptive immune system, has been adapted by scientists to enable site specific genome editing of eukaryotic cells both in vitro and in vivo. Previously, we reported the deve...

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Bibliographic Details
Main Authors: Namrata Kumar, William Stanford, Christopher de Solis, Aradhana, Nigel D. Abraham, Trieu-Mi J. Dao, Sadiqa Thaseen, Anusha Sairavi, Cuauhtemoc Ulises Gonzalez, Jonathan E. Ploski
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-11-01
Series:Frontiers in Molecular Neuroscience
Subjects:
AAV vectors
CRISPR/Cas9
SaCas9
Cre recombinase
inducible promoter
doxycycline
Online Access:https://www.frontiersin.org/article/10.3389/fnmol.2018.00413/full

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