A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification

A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification

The objective of the present work was to develop a simple and sensitive radioenzymatic assay to quantify lysophosphatidic acid (LPA). For that, a recombinant rat LPA acid acyltransferase (LPAAT) produced in Escherichia coli was used. In the presence of [14C]oleoyl-CoA, LPAAT selectively catalyzes th...

Full description

Bibliographic Details
Main Authors:	Jean Sébastien Saulnier-Blache, Alexia Girard, Marie-Françoise Simon, Max Lafontan, Philippe Valet
Format:	Article
Language:	English
Published:	Elsevier 2000-12-01
Series:	Journal of Lipid Research
Subjects:	radioenzymatic detection lysophosphatidic acid acyltransferase phosphatidic acid serum plasma [14C]oleoyl-CoA
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520323555

Similar Items

The Structure and Function of Acylglycerophosphate Acyltransferase 4/ Lysophosphatidic Acid Acyltransferase Delta (AGPAT4/LPAATδ)
by: Mikhail A. Zhukovsky, et al.
Published: (2019-08-01)

Phosphatidic Acid Stimulates Myoblast Proliferation through Interaction with LPA1 and LPA2 Receptors
by: Ana Gomez-Larrauri, et al.
Published: (2021-02-01)

Co-expressing Eranthis hyemalis lysophosphatidic acid acyltransferase 2 and elongase improves two very long chain polyunsaturated fatty acid production in Brassica carinata
by: Dauenpen Meesapyodsuk, et al.
Published: (2021-06-01)

Substrate specificity of lysophosphatidic acid acyltransferase β—evidence from membrane and whole cell assays
by: David Hollenback, et al.
Published: (2006-03-01)

Studies of lysophosphatidic acid acyltransferases generating membrane lipid diversity in bacteria
by: Toyotake, Yosuke
Published: (2019)

PrLPAAT4, a Putative Lysophosphatidic Acid Acyltransferase from Paeonia rockii, Plays an Important Role in Seed Fatty Acid Biosynthesis
by: Qingyu Zhang, et al.
Published: (2017-10-01)

Hepatic triacylglycerol accumulation and insulin resistance
by: Cynthia A. Nagle, et al.
Published: (2009-01-01)

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans
by: Camilo F. Muñoz, et al.
Published: (2019-11-01)

Characterization of mouse lysophosphatidic acid acyltransferase 3: an enzyme with dual functions in the testis1[S]
by: Koichi Yuki, et al.
Published: (2009-05-01)

A Novel Lysophosphatidic Acid Acyltransferase of <i>Escherichia coli</i> Produces Membrane Phospholipids with a <i>cis</i>-vaccenoyl Group and Is Related to Flagellar Formation
by: Yosuke Toyotake, et al.
Published: (2020-05-01)

Increased DHA Production in Seed Oil Using a Selective Lysophosphatidic Acid Acyltransferase
by: Pushkar Shrestha, et al.
Published: (2018-08-01)

Lysophosphatidic Acid Signaling in Diabetic Nephropathy
by: Jong Han Lee, et al.
Published: (2019-06-01)

Increased triacylglycerol production in oleaginous microalga Neochloris oleoabundans by overexpression of plastidial lysophosphatidic acid acyltransferase
by: Wipa Chungjatupornchai, et al.
Published: (2019-03-01)

Identification of Rice Koji Extract Components that Increase β-Glucocerebrosidase Levels in Human Epidermal Keratinocytes
by: Kazuhisa Maeda, et al.
Published: (2018-06-01)

Elevated plasma levels of lysophosphatidic acid and aberrant expression of lysophosphatidic acid receptors in adenomyosis
by: Bicheng Yang, et al.
Published: (2017-11-01)

Lysophosphatidic Acid and Hematopoiesis: From Microenvironmental Effects to Intracellular Signaling
by: Kuan-Hung Lin, et al.
Published: (2020-03-01)

Lysophosphatidic acid species are associated with exacerbation in chronic obstructive pulmonary disease
by: Qingling Li, et al.
Published: (2021-09-01)

Lysophosphatidic Acid Receptor Antagonists and Cancer: The Current Trends, Clinical Implications, and Trials
by: Yu-Hsuan Lin, et al.
Published: (2021-06-01)

Supplementation of Culture Media with Lysophosphatidic Acid Improves The Follicular Development of Human Ovarian Tissue after Xenotransplantaion into The Back Muscle of γ-Irradiated Mice
by: Zeynab Mohammadi, et al.
Published: (2020-10-01)

Dual Role of Lysophosphatidic Acid Receptor 2 (LPA2) in Amyotrophic Lateral Sclerosis
by: Maria Puigdomenech-Poch, et al.
Published: (2021-03-01)

Cytosolic Lysophosphatidic Acid Acyltransferase : Implications in Lipid Biosynthesis in Yeast, Plants and Human
by: Ghosh, Ananda Kumar
Published: (2018)

Autotaxin–Lysophosphatidic Acid Signaling in Alzheimer’s Disease
by: Sindhu Ramesh, et al.
Published: (2018-06-01)

Regulation of Tumor Immunity by Lysophosphatidic Acid
by: Sue Chin Lee, et al.
Published: (2020-05-01)

Lysophosphatidic Acid and Autotaxin-associated Effects on the Initiation and Progression of Colorectal Cancer
by: C. Chris Yun
Published: (2019-07-01)

Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga <i>Cyanidioschyzon merolae</i>
by: Sota Takahashi, et al.
Published: (2021-05-01)

Synthesis of an unsaturated fatty acid analogue (18-(4′-azido-2′-hydroxybenzoylamino)-oleic acid) and its interaction with lysophosphatidylcholine: acyl-CoA-O-acyltransferase
by: Lars Gehring, et al.
Published: (1998-05-01)

Lysophosphatidic Acid Is a Proinflammatory Stimulus of Renal Tubular Epithelial Cells
by: Aidinis, V., et al.
Published: (2022)

Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum
by: Brian De La Franier, et al.
Published: (2020-02-01)

Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed
by: Grace Q. Chen, et al.
Published: (2016-04-01)

Profiling lysophosphatidic acid levels in plasma from head and neck cancer patients
by: Mariati Abdul Rahman, et al.
Published: (2020-06-01)

Autotaxin promotes cancer cell invasion via the lysophosphatidic acid receptor 4
by: Harper, Kelly
Published: (2010)

Lysophosphatidic Acid Signaling in Cancer Cells: What Makes LPA So Special?
by: Pravita Balijepalli, et al.
Published: (2021-08-01)

Lysophosphatidic acid (LPA) 18:1 transcriptional regulation of primary human gingival fibroblasts
by: D. Roselyn Cerutis, et al.
Published: (2014-12-01)

TAZ Mediates Lysophosphatidic Acid-Induced Migration and Proliferation of Epithelial Ovarian Cancer Cells
by: Geun Ok Jeong, et al.
Published: (2013-07-01)

Lysophosphatidic Acid Promotes Epithelial to Mesenchymal Transition in Ovarian Cancer Cells by Repressing SIRT1
by: Upasana Ray, et al.
Published: (2017-02-01)

Polydopamine-Lysophosphatidate-Functionalised Titanium: A Novel Hybrid Surface Finish for Bone Regenerative Applications
by: Fiona Baldwin, et al.
Published: (2020-03-01)

A Novel Complementation Assay for Quick and Specific Screen of Genes Encoding Glycerol-3-Phosphate Acyltransferases
by: Jie Lei, et al.
Published: (2018-03-01)

LPAAT3 incorporates docosahexaenoic acid into skeletal muscle cell membranes and is upregulated by PPARδ activation
by: William J. Valentine, et al.
Published: (2018-02-01)

Synergistic Effect of WTC-Particulate Matter and Lysophosphatidic Acid Exposure and the Role of RAGE: In-Vitro and Translational Assessment
by: Rachel Lam, et al.
Published: (2020-06-01)

Convergence of vitamin D and lysophosphatidic acid signalling in stimulating human osteoblast maturation.
by: Jason Peter Mansell
Published: (2014-07-01)