Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
Abstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring wit...
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doaj-bfe5af5d5540450190029b0c190d50062021-07-25T11:28:42ZengBMCBiotechnology for Biofuels1754-68342021-07-0114111110.1186/s13068-021-02011-yEnhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mappingTangwu Cui0Bo Yuan1Haiwei Guo2Hua Tian3Weimin Wang4Yingqun Ma5Changzhi Li6Qiang Fei7School of Chemical Engineering and Technology, Xi’an Jiaotong UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityCAS Key Laboratory of Science and Technology On Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDepartment of Chemistry, Pennsylvania State UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityCAS Key Laboratory of Science and Technology On Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of SciencesSchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityAbstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. Results In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.https://doi.org/10.1186/s13068-021-02011-yWhite rot fungi (WRF)Lignin biodegradationLaccaseManganese peroxidaseSynergistic effectProduct mapping |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tangwu Cui Bo Yuan Haiwei Guo Hua Tian Weimin Wang Yingqun Ma Changzhi Li Qiang Fei |
spellingShingle |
Tangwu Cui Bo Yuan Haiwei Guo Hua Tian Weimin Wang Yingqun Ma Changzhi Li Qiang Fei Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping Biotechnology for Biofuels White rot fungi (WRF) Lignin biodegradation Laccase Manganese peroxidase Synergistic effect Product mapping |
author_facet |
Tangwu Cui Bo Yuan Haiwei Guo Hua Tian Weimin Wang Yingqun Ma Changzhi Li Qiang Fei |
author_sort |
Tangwu Cui |
title |
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
title_short |
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
title_full |
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
title_fullStr |
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
title_full_unstemmed |
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
title_sort |
enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2021-07-01 |
description |
Abstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. Results In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization. |
topic |
White rot fungi (WRF) Lignin biodegradation Laccase Manganese peroxidase Synergistic effect Product mapping |
url |
https://doi.org/10.1186/s13068-021-02011-y |
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