Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping

Abstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring wit...

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Main Authors: Tangwu Cui, Bo Yuan, Haiwei Guo, Hua Tian, Weimin Wang, Yingqun Ma, Changzhi Li, Qiang Fei
Format: Article
Language:English
Published: BMC 2021-07-01
Series:Biotechnology for Biofuels
Subjects:
Online Access:https://doi.org/10.1186/s13068-021-02011-y
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spelling doaj-bfe5af5d5540450190029b0c190d50062021-07-25T11:28:42ZengBMCBiotechnology for Biofuels1754-68342021-07-0114111110.1186/s13068-021-02011-yEnhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mappingTangwu Cui0Bo Yuan1Haiwei Guo2Hua Tian3Weimin Wang4Yingqun Ma5Changzhi Li6Qiang Fei7School of Chemical Engineering and Technology, Xi’an Jiaotong UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityCAS Key Laboratory of Science and Technology On Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of SciencesDepartment of Chemistry, Pennsylvania State UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversitySchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityCAS Key Laboratory of Science and Technology On Applied Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of SciencesSchool of Chemical Engineering and Technology, Xi’an Jiaotong UniversityAbstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. Results In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.https://doi.org/10.1186/s13068-021-02011-yWhite rot fungi (WRF)Lignin biodegradationLaccaseManganese peroxidaseSynergistic effectProduct mapping
collection DOAJ
language English
format Article
sources DOAJ
author Tangwu Cui
Bo Yuan
Haiwei Guo
Hua Tian
Weimin Wang
Yingqun Ma
Changzhi Li
Qiang Fei
spellingShingle Tangwu Cui
Bo Yuan
Haiwei Guo
Hua Tian
Weimin Wang
Yingqun Ma
Changzhi Li
Qiang Fei
Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
Biotechnology for Biofuels
White rot fungi (WRF)
Lignin biodegradation
Laccase
Manganese peroxidase
Synergistic effect
Product mapping
author_facet Tangwu Cui
Bo Yuan
Haiwei Guo
Hua Tian
Weimin Wang
Yingqun Ma
Changzhi Li
Qiang Fei
author_sort Tangwu Cui
title Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
title_short Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
title_full Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
title_fullStr Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
title_full_unstemmed Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
title_sort enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping
publisher BMC
series Biotechnology for Biofuels
issn 1754-6834
publishDate 2021-07-01
description Abstract Background As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin degradation, biological conversion is a promising approach featuring with mild conditions and diversity, and has received great attention nowadays. Results In this study, a consortium of white rot fungi composed of Lenzites betulina and Trametes versicolor was employed to enhance the ligninolytic enzyme activity of laccase (Lac) and manganese peroxidase (MnP) under microbial synergism. The maximum enzymatic activity of Lac and MnP was individually 18.06 U mL−1 and 13.58 U mL−1 along with a lignin degradation rate of 50% (wt/wt), which were achieved from batch cultivation of the consortium. The activities of Lac and MnP obtained from the consortium were both improved more than 40%, as compared with monocultures of L. betulina or T. versicolor under the same culture condition. The enhanced biodegradation performance was in accordance with the results observed from scanning electron microscope (SEM) of lignin samples before and after biodegradation, and secondary-ion mass spectrometry (SIMS). Finally, the analysis of heteronuclear single quantum coherence (HSQC) NMR and gas chromatography–mass spectrometry (GC–MS) provided a comprehensive product mapping of the lignin biodegradation, suggesting that the lignin has undergone depolymerization of the macromolecules, side-chain cleavage, and aromatic ring-opening reactions. Conclusions Our results revealed a considerable escalation on the enzymatic activity obtained in a short period from the cultivation of the L. betulina or T. versicolor due to the enhanced microbial synergistic effects, providing a potential bioconversion route for lignin utilization.
topic White rot fungi (WRF)
Lignin biodegradation
Laccase
Manganese peroxidase
Synergistic effect
Product mapping
url https://doi.org/10.1186/s13068-021-02011-y
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