Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study

Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study

A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC...

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Main Authors: Roman Kand’ár, Pavla Novotná, Petra Drábková
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2013/460242
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spelling doaj-bfd02a19caf947f5a9fed1e7500491dc2020-11-24T22:03:22ZengHindawi LimitedJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/460242460242Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical StudyRoman Kand’ár0Pavla Novotná1Petra Drábková2Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, 53210 Pardubice, Czech RepublicDepartment of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, 53210 Pardubice, Czech RepublicDepartment of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, 53210 Pardubice, Czech RepublicA method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC system. The separation was realized on an analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interassay coefficients of variation were below 10%. The recoveries were as follows: 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for α-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for β-carotene. The levels of selected fat-soluble vitamins in plasma of patients with cardiovascular disease were measured and discussed.http://dx.doi.org/10.1155/2013/460242
collection DOAJ
language English
format Article
sources DOAJ
author Roman Kand’ár
Pavla Novotná
Petra Drábková
spellingShingle Roman Kand’ár
Pavla Novotná
Petra Drábková
Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
Journal of Chemistry
author_facet Roman Kand’ár
Pavla Novotná
Petra Drábková
author_sort Roman Kand’ár
title Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
title_short Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
title_full Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
title_fullStr Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
title_full_unstemmed Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
title_sort determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma using hplc with uv-vis detection: application to a clinical study
publisher Hindawi Limited
series Journal of Chemistry
issn 2090-9063
2090-9071
publishDate 2013-01-01
description A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directly into the HPLC system. The separation was realized on an analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interassay coefficients of variation were below 10%. The recoveries were as follows: 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for α-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for β-carotene. The levels of selected fat-soluble vitamins in plasma of patients with cardiovascular disease were measured and discussed.
url http://dx.doi.org/10.1155/2013/460242
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AT pavlanovotna determinationofretinolatocopherollycopeneandbcaroteneinhumanplasmausinghplcwithuvvisdetectionapplicationtoaclinicalstudy
AT petradrabkova determinationofretinolatocopherollycopeneandbcaroteneinhumanplasmausinghplcwithuvvisdetectionapplicationtoaclinicalstudy
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