Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin

• Main Authors: Marino Gennaro, Giuliani Maria, Parrilli Ermenegilda, Tutino Maria
• Format: Article
• Language: English
• Published: BMC 2010-03-01
• Series: Microbial Cell Factories
• Online Access: http://www.microbialcellfactories.com/content/9/1/19

<p>Abstract</p> <p>Background</p> <p>Protein over-production in <it>Escherichia coli </it>often results in formation of inclusion bodies (IBs). Some recent reports have shown that the aggregation into IBs does not necessarily mean that the target protein is inactivated and that IBs may contain a high proportion of correctly folded protein. This proportion is variable depending on the protein itself, the genetic background of the producing cells and the expression temperature. In this paper we have evaluated the influence of other production process parameters on the quality of an inclusion bodies protein.</p> <p>Results</p> <p>The present paper describes the recombinant production in <it>Escherichia coli </it>of the flavohemoglobin from the Antarctic bacterium <it>Pseudoalteromonas haloplanktis </it>TAC125. Flavohemoglobins are multidomain proteins requiring FAD and heme cofactors. The production was carried out in several different experimental setups differing in bioreactor geometry, oxygen supply and the presence of a nitrosating compound. In all production processes, the recombinant protein accumulates in IBs, from which it was solubilized in non-denaturing conditions. Comparing structural properties of the solubilized flavohemoglobins, i.e. deriving from the different process designs, our data demonstrated that the protein preparations differ significantly in the presence of cofactors (heme and FAD) and as far as their secondary and tertiary structure content is concerned.</p> <p>Conclusions</p> <p>Data reported in this paper demonstrate that other production process parameters, besides growth temperature, can influence the structure of a recombinant product that accumulates in IBs. To the best of our knowledge, this is the first reported example in which the structural properties of a protein solubilized from inclusion bodies have been correlated to the production process design.</p>