Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells

Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new la...

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Main Authors: Qian Liu, Yaxi Zhu, Peter C. Amadio, Steven L. Moran, Anne Gingery, Chunfeng Zhao
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2018/3697971
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spelling doaj-bfad640809a7405a8f0f6d68b88665e72020-11-24T23:17:01ZengHindawi LimitedStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/36979713697971Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem CellsQian Liu0Yaxi Zhu1Peter C. Amadio2Steven L. Moran3Anne Gingery4Chunfeng Zhao5Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Molecular Medicine, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USADepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USATendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey. TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density. Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing. These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45. There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin. Turkey TDSCs also expressed transcription factors PouV, Nanog, and Sox2, which are critically involved in the regulation of stemness. The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model.http://dx.doi.org/10.1155/2018/3697971
collection DOAJ
language English
format Article
sources DOAJ
author Qian Liu
Yaxi Zhu
Peter C. Amadio
Steven L. Moran
Anne Gingery
Chunfeng Zhao
spellingShingle Qian Liu
Yaxi Zhu
Peter C. Amadio
Steven L. Moran
Anne Gingery
Chunfeng Zhao
Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
Stem Cells International
author_facet Qian Liu
Yaxi Zhu
Peter C. Amadio
Steven L. Moran
Anne Gingery
Chunfeng Zhao
author_sort Qian Liu
title Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
title_short Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
title_full Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
title_fullStr Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
title_full_unstemmed Isolation and Characterization of Multipotent Turkey Tendon-Derived Stem Cells
title_sort isolation and characterization of multipotent turkey tendon-derived stem cells
publisher Hindawi Limited
series Stem Cells International
issn 1687-966X
1687-9678
publishDate 2018-01-01
description Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey. TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density. Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing. These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45. There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin. Turkey TDSCs also expressed transcription factors PouV, Nanog, and Sox2, which are critically involved in the regulation of stemness. The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model.
url http://dx.doi.org/10.1155/2018/3697971
work_keys_str_mv AT qianliu isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
AT yaxizhu isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
AT petercamadio isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
AT stevenlmoran isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
AT annegingery isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
AT chunfengzhao isolationandcharacterizationofmultipotentturkeytendonderivedstemcells
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