Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells

Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have b...

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Main Authors: Hai-Tao Luo, Yuan-Yuan Zheng, Jun Tang, Li-Juan Shao, Yi-Heng Mao, Wei Yang, Xiao-Fei Yang, Yang Li, Rui-Jun Tian, Fu-Rong Li
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:npj Genomic Medicine
Online Access:https://doi.org/10.1038/s41525-021-00217-5
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spelling doaj-bf853ecf67ca4e85834b4772f5f78fed2021-06-20T11:46:05ZengNature Publishing Groupnpj Genomic Medicine2056-79442021-06-016111410.1038/s41525-021-00217-5Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cellsHai-Tao Luo0Yuan-Yuan Zheng1Jun Tang2Li-Juan Shao3Yi-Heng Mao4Wei Yang5Xiao-Fei Yang6Yang Li7Rui-Jun Tian8Fu-Rong Li9Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan UniversityTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Department of Chemistry, Southern University of Science and TechnologyTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Department of Chemistry, Southern University of Science and TechnologyTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.https://doi.org/10.1038/s41525-021-00217-5
collection DOAJ
language English
format Article
sources DOAJ
author Hai-Tao Luo
Yuan-Yuan Zheng
Jun Tang
Li-Juan Shao
Yi-Heng Mao
Wei Yang
Xiao-Fei Yang
Yang Li
Rui-Jun Tian
Fu-Rong Li
spellingShingle Hai-Tao Luo
Yuan-Yuan Zheng
Jun Tang
Li-Juan Shao
Yi-Heng Mao
Wei Yang
Xiao-Fei Yang
Yang Li
Rui-Jun Tian
Fu-Rong Li
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
npj Genomic Medicine
author_facet Hai-Tao Luo
Yuan-Yuan Zheng
Jun Tang
Li-Juan Shao
Yi-Heng Mao
Wei Yang
Xiao-Fei Yang
Yang Li
Rui-Jun Tian
Fu-Rong Li
author_sort Hai-Tao Luo
title Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
title_short Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
title_full Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
title_fullStr Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
title_full_unstemmed Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
title_sort dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
publisher Nature Publishing Group
series npj Genomic Medicine
issn 2056-7944
publishDate 2021-06-01
description Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.
url https://doi.org/10.1038/s41525-021-00217-5
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