Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells
Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have b...
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doaj-bf853ecf67ca4e85834b4772f5f78fed2021-06-20T11:46:05ZengNature Publishing Groupnpj Genomic Medicine2056-79442021-06-016111410.1038/s41525-021-00217-5Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cellsHai-Tao Luo0Yuan-Yuan Zheng1Jun Tang2Li-Juan Shao3Yi-Heng Mao4Wei Yang5Xiao-Fei Yang6Yang Li7Rui-Jun Tian8Fu-Rong Li9Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan UniversityTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Department of Chemistry, Southern University of Science and TechnologyTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Translational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Department of Chemistry, Southern University of Science and TechnologyTranslational Medicine Collaborative Innovation Center, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology)Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.https://doi.org/10.1038/s41525-021-00217-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hai-Tao Luo Yuan-Yuan Zheng Jun Tang Li-Juan Shao Yi-Heng Mao Wei Yang Xiao-Fei Yang Yang Li Rui-Jun Tian Fu-Rong Li |
spellingShingle |
Hai-Tao Luo Yuan-Yuan Zheng Jun Tang Li-Juan Shao Yi-Heng Mao Wei Yang Xiao-Fei Yang Yang Li Rui-Jun Tian Fu-Rong Li Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells npj Genomic Medicine |
author_facet |
Hai-Tao Luo Yuan-Yuan Zheng Jun Tang Li-Juan Shao Yi-Heng Mao Wei Yang Xiao-Fei Yang Yang Li Rui-Jun Tian Fu-Rong Li |
author_sort |
Hai-Tao Luo |
title |
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
title_short |
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
title_full |
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
title_fullStr |
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
title_full_unstemmed |
Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
title_sort |
dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells |
publisher |
Nature Publishing Group |
series |
npj Genomic Medicine |
issn |
2056-7944 |
publishDate |
2021-06-01 |
description |
Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development. |
url |
https://doi.org/10.1038/s41525-021-00217-5 |
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