The proliferation of cervical cancer is promoted by miRNA-125b through the regulation of the HMGA1

• Main Authors: Sun B, Zhang Y, Zhou L, Yin L, Li F, Li C, Xia J
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-04-01
• Series: OncoTargets and Therapy
• Subjects: Cervical cancer (CC); miR-125b; high mobility group A (HMGA1); progression free survival; overall survival (OS); prognosis (PFS)
• Online Access: https://www.dovepress.com/the-proliferation-of-cervical-cancer-is-promoted-by-microrna-125b-thro-peer-reviewed-article-OTT

Bingmei Sun, Ying Zhang, Lianxiang Zhou, Linin Yin, Fei Li, Chao Li, Jiayu Xia Department of Gynaecology and Obstetrics, Linyi Central Hospital, Linyi 276400, Shandong Province, China Background: It has been reported that miRNA-125b is associated with carcinogenesis and development of several different kinds of cancers. Nevertheless, there is no clarity regarding the significance and mechanism of action of miR-125b in clinical practice for cervical cancer (CC). Materials and methods: In the current investigation, the expression of miR-125b in cervical clinical specimens and CC cell lines was analyzed via real-time quantitative PCR, and the relationship of miR-125b with the chromatin-associated protein high mobility group A (HMGA1) expression and clinicopathological parameters of CC patients was explored. Results: The results indicated that miR-125b expression was remarkably upregulated in CC cell lines as well as in the tissues of humans. miR-125b overexpression was significantly related to a decrease in HMGA1 expression, progression-free survival, overall survival, and prognosis as well. Besides, knockdown of miR-125b inhibited proliferation and colony formation in SW756 and C4-1 cells, where the 3'-UTR of HMGA1 mRNA was directly targeted. Moreover, PI3K/Akt pathway was regulated by miR-125b through suppression of HMGA1. Conclusion: These findings illustrated that a new regulatory role of HMGA1 is involved in the progression of CC. Our data demonstrated that miR-125b could play a critical role in the carcinogenesis and progression of CC, revealing that miR-125b might serve as a potential new target for treating CC. Keywords: cervical cancer, CC, miR-125b, high mobility group A, HMGA1, progression-free survival, PFS, overall survival, OS, prognosis