Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors.

Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors.

Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dyn...

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Bibliographic Details
Main Authors: Jolien Vermeire, Evelien Naessens, Hanne Vanderstraeten, Alessia Landi, Veronica Iannucci, Anouk Van Nuffel, Tom Taghon, Massimo Pizzato, Bruno Verhasselt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3515444?pdf=render

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