Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography

Background: To establish a fast and simple quantitative method for detection of heart-type fatty acid-binding protein (H-FABP) in serum based on a background fluorescence quenching im munochromatographic assay. Methods: A detection card based on the double-antibody sandwich double-antibody method wi...

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Main Authors: Zhang Wei, Chen Junlei, Li Xinxia, Wang Yuwen, Li Jiutong
Format: Article
Language:English
Published: Society of Medical Biochemists of Serbia, Belgrade 2019-01-01
Series:Journal of Medical Biochemistry
Subjects:
Online Access:https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902172Z.pdf
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spelling doaj-bf6dee0aee4e40948bdb1d0195e987cc2020-11-25T03:53:20ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662019-01-013821721801452-82581902172ZDevelopment of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatographyZhang Wei0Chen Junlei1Li Xinxia2Wang Yuwen3Li Jiutong4Xinjiang Medical University, Pharmacy Academy, Urumqi, P. R. ChinaXinjiang Normal University, Chemistry and Chemical Engineering Academy, Urumqi, P. R. ChinaXinjiang Medical University, Pharmacy Academy, Urumqi, P. R. China + Ministry of Science and Technology and Xinjiang Uygur Autonomous Region,Urumqi, P. R. ChinaXinjiang uygur autonomous region center of disease control and prevention, Urumqi, P. R. ChinaShanghai Simp Bio-Science Co., Ltd., Shanghai, P. R. ChinaBackground: To establish a fast and simple quantitative method for detection of heart-type fatty acid-binding protein (H-FABP) in serum based on a background fluorescence quenching im munochromatographic assay. Methods: A detection card based on the double-antibody sandwich double-antibody method with background fluorescence quenching was developed for quantitative m easurement of H-FABP in serum. The optimal concentrations of control for coating the test and control lines were determined as well as the concentrations of gold-labeled antibodies used in preparing the detection system. The detection method for H-FABP in serum was established and validated using real-world clinical samples. Results: The optimal concentrations of labeling antibody and coating antibody were 5.0 mg/mL and 1.0 mg/m L, respectively. The test card had a sensitivity of 1.15 ng/m L over a linear concentration range of 0-100 ng/m L. Based on three batches prepared for testing the card, the relative standard deviation (RSD) within batches was less than 15% without a significant difference (P = 0.942 ). The detection method was tested against common interfering substances in serum, such as bilirubin, triglyceride and serum anticoagulants ethylenediamine tetraacetic acid (EDTA), heparin, and sodium citrate, and no significant cross-reaction was detected. The test method was further validated with 50 clinical serum samples, and the test results were COM parable with standard reference detection methods with good correlation (R = 0.95 ). Conclusion: O ur study presents a new method with strong specificity and sensitivity for the detection of H-FABP in serum, which could promote H-FABP detection in a broad range of applications.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902172Z.pdfheart-type fatty acid binding proteinbackground fluorescence quenchingimmunochromatography
collection DOAJ
language English
format Article
sources DOAJ
author Zhang Wei
Chen Junlei
Li Xinxia
Wang Yuwen
Li Jiutong
spellingShingle Zhang Wei
Chen Junlei
Li Xinxia
Wang Yuwen
Li Jiutong
Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
Journal of Medical Biochemistry
heart-type fatty acid binding protein
background fluorescence quenching
immunochromatography
author_facet Zhang Wei
Chen Junlei
Li Xinxia
Wang Yuwen
Li Jiutong
author_sort Zhang Wei
title Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
title_short Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
title_full Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
title_fullStr Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
title_full_unstemmed Development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
title_sort development of a quantitative detection card for heart-type fatty acid-binding protein based on background fluorescence quenching immune chromatography
publisher Society of Medical Biochemists of Serbia, Belgrade
series Journal of Medical Biochemistry
issn 1452-8258
1452-8266
publishDate 2019-01-01
description Background: To establish a fast and simple quantitative method for detection of heart-type fatty acid-binding protein (H-FABP) in serum based on a background fluorescence quenching im munochromatographic assay. Methods: A detection card based on the double-antibody sandwich double-antibody method with background fluorescence quenching was developed for quantitative m easurement of H-FABP in serum. The optimal concentrations of control for coating the test and control lines were determined as well as the concentrations of gold-labeled antibodies used in preparing the detection system. The detection method for H-FABP in serum was established and validated using real-world clinical samples. Results: The optimal concentrations of labeling antibody and coating antibody were 5.0 mg/mL and 1.0 mg/m L, respectively. The test card had a sensitivity of 1.15 ng/m L over a linear concentration range of 0-100 ng/m L. Based on three batches prepared for testing the card, the relative standard deviation (RSD) within batches was less than 15% without a significant difference (P = 0.942 ). The detection method was tested against common interfering substances in serum, such as bilirubin, triglyceride and serum anticoagulants ethylenediamine tetraacetic acid (EDTA), heparin, and sodium citrate, and no significant cross-reaction was detected. The test method was further validated with 50 clinical serum samples, and the test results were COM parable with standard reference detection methods with good correlation (R = 0.95 ). Conclusion: O ur study presents a new method with strong specificity and sensitivity for the detection of H-FABP in serum, which could promote H-FABP detection in a broad range of applications.
topic heart-type fatty acid binding protein
background fluorescence quenching
immunochromatography
url https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581902172Z.pdf
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AT lixinxia developmentofaquantitativedetectioncardforhearttypefattyacidbindingproteinbasedonbackgroundfluorescencequenchingimmunechromatography
AT wangyuwen developmentofaquantitativedetectioncardforhearttypefattyacidbindingproteinbasedonbackgroundfluorescencequenchingimmunechromatography
AT lijiutong developmentofaquantitativedetectioncardforhearttypefattyacidbindingproteinbasedonbackgroundfluorescencequenchingimmunechromatography
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