Antibiotic resistance of Salmonella enterica producing Extended-spectrum B-lactamases (ESBLs) type CMY-2, in poultry

• Main Authors: Maryam Naderi Mozajin, Pejvak Khaki, Fatemeh Noorbakhsh
• Format: Article
• Language: fas
• Published: Golestan University of Medical Sciences 2018-05-01
• Series: مجله دانشگاه علوم پزشکی گرگان
• Subjects: Salmonella enterica; ESBL; Multi-drug resistant; Bala CMY2 gene
• Online Access: http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1036&slc_lang=en&sid=1
• ISSN: 1562-4765; 2008-4080

Background and Objective: Salmonella is one of the most important zoonotic pathogens responsible for food-borne infections all over the world. Poultry products are widely acknowledged to be a significant reservoir for Salmonella. This study was done to evaluate the antibiotic resistant of Salmonella enterica producer of beta lactamase spectrum in poultry. Methods: In this descriptive – laboratort study 70 Salmonella enterica serotypes were collected from poultry. All Salmonella isolates were tested to antimicrobial susceptibility testing by the Kirby-Bauer disk diffusion according to Clinical Laboratory Standards Institute (CLSI). Twenty-nine antibiotics were used in this study. Klebsiella pneumoniae; ATCC 700603 was used as quality control strains. The isolates were determined to be ESBL-producing Salmonella by the conventional double-disk synergy and genotypic method. Results: Among 70 salmonella isolates, the most prevalent serotypes were S.typhimurium and S.enteritidis. All serotypes were susceptible to gentamicin, ciprofloxacin, oflaxacin, imipenem, enrofloxacin. The common resistance was observed to cephalexin (96%), cefazolin (96%) and cephalotin (65%). Among the 70 Salmonella isolates studied, multi-drug resistance was observed in 59 (84%) isolates. Forty-seven (67%) isolates were found to be ESBL-producing isolates. PCR assay of all isolates showed that 17 isolates (33.3%) carried bala CMY2 gene. Conclusion: This study showed that antibiotic resistance to Salmonella enterica serotypes is due to beta lactamase enzyme in this strain is considerably increased in poultry.