Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil

• Main Authors: Elmary da Costa Fraga, Joselita Maria Mendes dos Santos, Juracy de Freitas Maia
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Genética 2003-01-01
• Series: Genetics and Molecular Biology
• Subjects: Aedes aegypti; electrophoreses; isozymes; populations genetics
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200011

Eighteen enzymatic loci were analysed in Aedes aegypti populations from four neighbourhoods in the city of Manaus. The analyses showed that the Downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (Ho = 0.152 ± 0.052; He = 0.174 ± 0.052, respectively). The least variability was detected in the Coroado and Cidade Nova populations, both with polymorphism of 44.4%. The latter population presented the least observed heterozygosity (Ho = 0.109 ± 0.037). Wright's F statistics showed that the mean value of Fis was higher than that of Fst (Fis = 0.164 > Fst = 0.048), and from analysis of molecular variance (AMOVA) it was found that 95.12% of the variability is found within populations indicating a certain intra-population differentiation possibly of the microgeographic structure resulting from some barrier in the random coupling. Although the four populations were similar genetically (D = 0.003 to 0.016), the 4.88% differentiation was significant.