T1N6_22 positively regulates Botrytis cinerea resistance but negatively regulates Pseudomonas syringae pv. tomato DC3000 resistance in Arabidopsis thaliana

• Main Authors: Qiao-yun Weng, Jin-hui Song, Ya-ting Zhao, Xu Zheng, Cong-cong Huang, Guan-yu Wang, Jing Zhang, Ji-hong Xing, Jin-gao Dong
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2017-07-01
• Series: Biotechnology & Biotechnological Equipment
• Subjects: Arabidopsis thaliana; T1N6_22; Botrytis cinerea; Pseudomonas syringae pv. tomato DC3000; salicylic acid; jasmonic acid; signalling pathways
• Online Access: http://dx.doi.org/10.1080/13102818.2017.1318717
• ISSN: 1310-2818; 1314-3530

T1N6_22, a short-chain dehydrogenase/reductase family protein, was identified as a positive regulator in Arabidopsis thaliana resistance against Botrytis cinerea and Alternaria brassicae in our preliminary study. In this study, we found that the expression levels of the T1N6_22 gene were induced and up-regulated in A. thaliana ecotype Columbia (Col-0) after B. cinerea and Pseudomonas syringae pv. tomato DC3000 inoculation. Compared with the Col-0 and t1n6_22/T1N6_22 plants, the expression of PAL, PR4, PPO, SOD and CAT genes were down-regulated in the t1n6_22 plants. In Col-0 plants treated with salicylic acid (SA) and the SA analogue benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH), the expression levels of T1N6_22 were significantly enhanced, whereas the expression levels of T1N6_22 were reduced by jasmonic acid treatment. Meanwhile, the t1n6_22 mutant exhibited enhanced resistance, whereas the wild-type Col-0 and complemented plants (t1n6_22/T1N6_22) showed susceptibility to Pst DC3000. After inoculation with B. cinerea and Pst DC3000, the expression levels of defence-related genes PR1, PR3, PR5, NPR1 and PDF1.2 in t1n6_22 were significantly different from those in Col-0 and t1n6_22/T1N6_22 plants. Taken together, the T1N6_22 gene played a negative role in Arabidopsis resistance to Pst DC3000. The T1N6_22 gene may be involved in the regulation of salicylic acid and jasmonic-acid–signalling pathways to affect the resistance of Arabidopsis to B. cinerea and Pst DC3000.