Astragaloside regulates the growth and invasion activity of colon cancer cells in vitro: the experimental study

• Main Authors: Qiang Li, Rong-Chi Mao, Hui Huang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2018-10-01
• Series: Journal of Hainan Medical University
• Subjects: Colon cancer; Astragaloside; Proliferation; Invasion
• Online Access: http://www.hnykdxxb.com/PDF/201819/01.pdf
• ISSN: 1007-1237; 1007-1237

Objective: To study the regulating effects of astragaloside on the growth and invasion activity of colon cancer cells in vitro. Methods: Lovo colon cancer cell lines were cultured and randomly divided into the control group treated with DMEM without drug or serum and the astragaloside groups treated with serum-free DMEM containing 10 μmol/L, 20 μmol/L and 40 μmol/L astragaloside. The cell proliferation activity, the number of invasive cells as well as the expression of proliferating genes and invasion genes were measured after 24 h of treatment. Results: 24 h after treatment, the cell proliferation activity, the number of invasive cells as well as CCND1, β-catenin, ADAM17, MMP11, VEGF and ZEB2 mRNA expression of different concentrations of astragaloside groups were obviously lower than those of blank control group while WTX, PERK, ATF6, TIMP1 and E-cadherin mRNA expression were significantly higher than those of blank control group, and the higher the concentration of astragaloside, the more significant the decreasing trend of the cell proliferation activity, the number of invasive cells as well as CCND1, β-catenin, ADAM17, MMP11, VEGF and ZEB2 mRNA expression and the increasing trend of the WTX, PERK, ATF6, TIMP1 and E-cadherin mRNA expression. Conclusion: Astragaloside can significantly inhibit the growth and invasion of colon cancer cells in vitro.