Detection of Trichomoniasis in cattle in Nineveh province

• Main Authors: Wasan A. Alobaidii, Qaes T. Alobaidii, Sadam D. Hassan
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2021-02-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: tritrichomonas foetus; cow; clinical status; cpcr
• Online Access: https://vetmedmosul.com/article_167614_559f71a4a79f5d2806f245b957866599.pdf

The current study was conducted on the detection of <em>Tritrichomonas foetus</em> in vaginal mucus of infected cows using conventional polymerase chain reaction technique (cPCR) in Nineveh province, Iraq. A total of 87 vaginal mucus samples were collected randomly from the vagina of Heifer cows of different ages (2-4, >4-6, >6 years old) and stages of pregnancy with different clinical status (early embryonic death, pyometra, abortion and healthy animals once) by washing cow’s vagina using artificial insemination pipette, DNA extraction of <em>T. foetus</em> was done from vaginal mucus samples, cPCR was attempt using TFR3 and TFR4 primers, Results indicated that 11 cows (12.6 %) were positive for <em>T. foetus</em>. The clinical status of cows demonstrated statistically significant (P<0.05) a higher percentage of Early Embryonic Death at (6.9%) compared to pyometra, abortion, and healthy cows. Furthermore, the percentage of <em>T. foetus</em>infection was significantly (P<0.05) elevated among cows (>2-4 years old) at (8%) compared to (>4-6 years old) and (>6 years old) cows. This study concluded that <em>T. foetus</em>infection was an elevated percentage of infection in cows with early embryonic death and in cows (>2-4) years old. This study is the first detected <em>T. foetus</em> in cattle in Nineveh province.