Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping

Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping

A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the metho...

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Main Authors: Randa Bittar, Alain Carrié, Georges Nouadje, Corinne Cherfils, Valérie Fesel-Fouquier, Laurence Barbot-Trystram, Philippe Giral, Dominique Bonnefont-Rousselot
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Practical Laboratory Medicine
Online Access:http://www.sciencedirect.com/science/article/pii/S2352551719301076
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spelling doaj-befe669d18fb49a0abf8550989c4a3d62020-11-25T00:29:30ZengElsevierPractical Laboratory Medicine2352-55172020-01-0118Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotypingRanda Bittar0Alain Carrié1Georges Nouadje2Corinne Cherfils3Valérie Fesel-Fouquier4Laurence Barbot-Trystram5Philippe Giral6Dominique Bonnefont-Rousselot7Service de Biochimie Métabolique, AP-HP.Sorbonne Université, F-75013, Paris, France; Sorbonne Université, UMR_S1166 ICAN, F-75013, Paris, FranceSorbonne Université, UMR_S1166 ICAN, F-75013, Paris, France; Service de Biochimie Endocrinienne et Oncologique, AP-HP.Sorbonne Université, F-75013, Paris, FranceSebia, F-91008, Evry, FranceService de Biochimie Métabolique, AP-HP.Sorbonne Université, F-75013, Paris, FranceService de Biochimie Métabolique, AP-HP.Sorbonne Université, F-75013, Paris, FranceService de Coprologie Fonctionnelle, AP-HP.Sorbonne Université, F-75013, Paris, FranceSorbonne Université, UMR_S1166 ICAN, F-75013, Paris, France; Service d’Endocrinologie-Métabolisme, AP-HP.Sorbonne Université, F-75013, Paris, FranceService de Biochimie Métabolique, AP-HP.Sorbonne Université, F-75013, Paris, France; Université de Paris, UTCBS, CNRS, INSERM, F-75006, Paris, France; Corresponding author. Service de Biochimie Métabolique, AP-HP.Sorbonne Université, Pitié-Salpêtrière-Charles Foix, 47-83 boulevard de l’Hôpital, F-75013, Paris, France.A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of this method to help clinicians in the diagnosis of dyslipidemic and neurodegenerative diseases. Keywords: Apolipoprotein E, Isoforms, Phenotype, Isoelectric focusinghttp://www.sciencedirect.com/science/article/pii/S2352551719301076
collection DOAJ
language English
format Article
sources DOAJ
author Randa Bittar
Alain Carrié
Georges Nouadje
Corinne Cherfils
Valérie Fesel-Fouquier
Laurence Barbot-Trystram
Philippe Giral
Dominique Bonnefont-Rousselot
spellingShingle Randa Bittar
Alain Carrié
Georges Nouadje
Corinne Cherfils
Valérie Fesel-Fouquier
Laurence Barbot-Trystram
Philippe Giral
Dominique Bonnefont-Rousselot
Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
Practical Laboratory Medicine
author_facet Randa Bittar
Alain Carrié
Georges Nouadje
Corinne Cherfils
Valérie Fesel-Fouquier
Laurence Barbot-Trystram
Philippe Giral
Dominique Bonnefont-Rousselot
author_sort Randa Bittar
title Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
title_short Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
title_full Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
title_fullStr Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
title_full_unstemmed Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping
title_sort evaluation of a semi-automatic isoelectric focusing method for apolipoprotein e phenotyping
publisher Elsevier
series Practical Laboratory Medicine
issn 2352-5517
publishDate 2020-01-01
description A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of this method to help clinicians in the diagnosis of dyslipidemic and neurodegenerative diseases. Keywords: Apolipoprotein E, Isoforms, Phenotype, Isoelectric focusing
url http://www.sciencedirect.com/science/article/pii/S2352551719301076
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