Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)

Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)

Plum is one of the most important stone fruits in the world and anthocyanin-rich plums are increasingly popular due to their health-promoting potential. In this study, we investigated the mechanisms of anthocyanin accumulation in the flesh of the red-fleshed mutant of the yellow-fleshed plum ‘Sanyue...

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Main Authors: Zhi-Zhen Fang, Kui Lin-Wang, Dan-Rong Zhou, Yan-Juan Lin, Cui-Cui Jiang, Shao-Lin Pan, Richard V. Espley, Christelle M. Andre, Xin-Fu Ye
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Plant Science
Subjects:
transcriptome
red-fleshed mutant
anthocyanin biosynthesis
PsMYB10.2
plum
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.680469/full
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spelling doaj-befc4ea6e7264dd0a74723d412eeec892021-06-22T14:54:23ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.680469680469Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)Zhi-Zhen Fang0Zhi-Zhen Fang1Kui Lin-Wang2Dan-Rong Zhou3Dan-Rong Zhou4Yan-Juan Lin5Yan-Juan Lin6Cui-Cui Jiang7Cui-Cui Jiang8Shao-Lin Pan9Shao-Lin Pan10Richard V. Espley11Christelle M. Andre12Xin-Fu Ye13Xin-Fu Ye14Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaThe New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, New ZealandFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaThe New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, New ZealandFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaFujian Engineering and Technology Research Center for Deciduous Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaPlum is one of the most important stone fruits in the world and anthocyanin-rich plums are increasingly popular due to their health-promoting potential. In this study, we investigated the mechanisms of anthocyanin accumulation in the flesh of the red-fleshed mutant of the yellow-fleshed plum ‘Sanyueli’. RNA-Seq and qRT-PCR showed that anthocyanin biosynthetic genes and the transcription factor PsMYB10.2 were upregulated in the flesh of the mutant. Functional testing in tobacco leaves indicated that PsMYB10.2 was an anthocyanin pathway activator and can activate the promoter of the anthocyanin biosynthetic genes PsUFGT and PsGST. The role of PsMYB10.2 in anthocyanin accumulation in the flesh of plum was further confirmed by virus-induced gene silencing. These results provide information for further elucidating the underlying mechanisms of anthocyanin accumulation in the flesh of plum and for the breeding of new red-fleshed plum cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2021.680469/fulltranscriptomered-fleshed mutantanthocyanin biosynthesisPsMYB10.2plum
collection DOAJ
language English
format Article
sources DOAJ
author Zhi-Zhen Fang
Zhi-Zhen Fang
Kui Lin-Wang
Dan-Rong Zhou
Dan-Rong Zhou
Yan-Juan Lin
Yan-Juan Lin
Cui-Cui Jiang
Cui-Cui Jiang
Shao-Lin Pan
Shao-Lin Pan
Richard V. Espley
Christelle M. Andre
Xin-Fu Ye
Xin-Fu Ye
spellingShingle Zhi-Zhen Fang
Zhi-Zhen Fang
Kui Lin-Wang
Dan-Rong Zhou
Dan-Rong Zhou
Yan-Juan Lin
Yan-Juan Lin
Cui-Cui Jiang
Cui-Cui Jiang
Shao-Lin Pan
Shao-Lin Pan
Richard V. Espley
Christelle M. Andre
Xin-Fu Ye
Xin-Fu Ye
Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
Frontiers in Plant Science
transcriptome
red-fleshed mutant
anthocyanin biosynthesis
PsMYB10.2
plum
author_facet Zhi-Zhen Fang
Zhi-Zhen Fang
Kui Lin-Wang
Dan-Rong Zhou
Dan-Rong Zhou
Yan-Juan Lin
Yan-Juan Lin
Cui-Cui Jiang
Cui-Cui Jiang
Shao-Lin Pan
Shao-Lin Pan
Richard V. Espley
Christelle M. Andre
Xin-Fu Ye
Xin-Fu Ye
author_sort Zhi-Zhen Fang
title Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
title_short Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
title_full Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
title_fullStr Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
title_full_unstemmed Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)
title_sort activation of psmyb10.2 transcription causes anthocyanin accumulation in flesh of the red-fleshed mutant of ‘sanyueli’ (prunus salicina lindl.)
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2021-06-01
description Plum is one of the most important stone fruits in the world and anthocyanin-rich plums are increasingly popular due to their health-promoting potential. In this study, we investigated the mechanisms of anthocyanin accumulation in the flesh of the red-fleshed mutant of the yellow-fleshed plum ‘Sanyueli’. RNA-Seq and qRT-PCR showed that anthocyanin biosynthetic genes and the transcription factor PsMYB10.2 were upregulated in the flesh of the mutant. Functional testing in tobacco leaves indicated that PsMYB10.2 was an anthocyanin pathway activator and can activate the promoter of the anthocyanin biosynthetic genes PsUFGT and PsGST. The role of PsMYB10.2 in anthocyanin accumulation in the flesh of plum was further confirmed by virus-induced gene silencing. These results provide information for further elucidating the underlying mechanisms of anthocyanin accumulation in the flesh of plum and for the breeding of new red-fleshed plum cultivars.
topic transcriptome
red-fleshed mutant
anthocyanin biosynthesis
PsMYB10.2
plum
url https://www.frontiersin.org/articles/10.3389/fpls.2021.680469/full
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