DEOXYNIVALENOL-INDUCED ANIMAL OVARIAN SIGNALING: PROLIFERATION AND APOPTOSIS

• Main Authors: Róbert Toman, Zuzana Baková, Nora Maruniaková, Marcela Capcarová, Adriana Kolesárová, Sushmita Nath, Alexander V. Sirotkin
• Format: Article
• Language: English
• Published: Slovak University of Agriculture 2011-12-01
• Series: Journal of Microbiology, Biotechnology and Food Sciences
• Subjects: Deoxynivalenol; IGF–I; apoptosis; ovary; rat
• Online Access: http://www.jmbfs.org/wp-content/uploads/2012/01/jmbfs_kolesarova_0034.pdf

Fusarium toxins stable natural toxins produced by Fusarium species can appear in the food chain. The aim of this in vitro study was to examine: 1. the effect of deoxynivalenol (DON) treatment on the ability of rat ovarian cells to release insulin-like growth factor I (IGF-I) and 2. a possible signaling pathway of cell apoptosis through selected markers (Bcl-2, Bax and caspase-3) induced by this natural toxin. Rat ovarian fragments were incubated with DON for 24h: 10, 100 and 1000 ng.ml-1, while the control group received no DON. The secretion of IGF–I was determined by RIA and expression of antiapoptotic (Bcl-2) and proapoptotic (Bax and caspase-3) proteins by Western-blotting analysis. IGF-I release by rat ovarian cells after DON addition was not significantly (P>0.05) stimulated (at the dose 10 ng.ml-1) and inhibited (at the doses 100 and 1000 ng.ml-1) by the toxin. The impact of DON on the expression of Bcl-2 (26 kDa), Bax (23 kDa) and caspase-3 (34 kDa) was found. Bcl-2 expression was decreased and Bax and caspase-3 increased by DON treatment. In conclusion, our results suggest a direct effect of DON on (1) ovarian functions, (2) mechanisms of proliferation and apoptosis in rat ovarian cells through intracellular regulators: Bcl-2, Bax and caspase-3.