Polysaccharide from tuberous roots of Ophiopogon japonicus regulates gut microbiota and its metabolites during alleviation of high-fat diet-induced type-2 diabetes in mice

• Main Authors: Huai-You Wang, Lin-Xiu Guo, Wei-Hui Hu, Zhi-Tian Peng, Cheng Wang, Zhi-Cong Chen, Etta Y.L. Liu, Tina T.X. Dong, Tie-Jie Wang, Karl W.K. Tsim
• Format: Article
• Language: English
• Published: Elsevier 2019-12-01
• Series: Journal of Functional Foods
• Subjects: Ophiopogon japonicus; Polysaccharides; Type-2 diabetes; Gut microbiota; Short-chain fatty acids
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464619305171

Ophiopogonis Radix (Maidong in Chinese), the tuberous roots of Ophiopogon japonicus having polysaccharide as the main active ingredient, is a traditional Chinese herb and popularly used as functional food additive in China. Here, a homogeneous polysaccharide fraction (OJP-W1) was isolated and characterized from Ophiopogonis Radix collected from Sichuan (Chuan-Maidong). The therapeutic effects of OJP-W1 in diabetes symptoms, gut microbiota and metabolism of short-chain fatty acids (SCFAs) in high-fat diet (HFD)-induced type-2 diabetes mice were investigated. Results showed that OJP-W1 significantly ameliorated HFD-induced insulin resistance and glucose tolerance. Meanwhile, OJP-W1 regulated the gut microbiota dysbiosis in HFD-fed mice, as indicated by increasing Actinobacteria and Bifidobacterium, decreasing Proteobacteria and type-2 diabetes-enriched taxa (e.g. Desulfovibrionaceae, Dorea and Ruminococcaceae). In addition, OJP-W1 could improve the metabolic disorder of SCFAs in HFD-fed mice. This study indicated that OJP-W1 might serve as a novel functional food in preventing diabetes-associated gut microbiota dysbiosis and SCFA metabolic disorder.