Renal tubular epithelial cells injury induced by mannitol and its potential mechanism

Administration of mannitol with high dose could induce extensive isometric renal proximal tubular vacuolization and acute renal failure in clinic. We previously demonstrated that mannitol-induced human kidney tubular epithelial cell (HK-2) injury. The objective of our present work was to further stu...

Full description

Bibliographic Details
Main Authors: Jinwan Shi, Jiuzhan Qian, Hui Li, Hongjun Luo, Wenhong Luo, Zhexuan Lin
Format: Article
Language:English
Published: Taylor & Francis Group 2018-10-01
Series:Renal Failure
Subjects:
Online Access:http://dx.doi.org/10.1080/0886022X.2017.1419973
id doaj-beaf639b986a4cb5be5ca518c382c303
record_format Article
spelling doaj-beaf639b986a4cb5be5ca518c382c3032020-11-25T02:04:45ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492018-10-01401859110.1080/0886022X.2017.14199731419973Renal tubular epithelial cells injury induced by mannitol and its potential mechanismJinwan Shi0Jiuzhan Qian1Hui Li2Hongjun Luo3Wenhong Luo4Zhexuan Lin5Shantou University Medical CollegeShantou University Medical CollegeShantou University Medical CollegeShantou University Medical CollegeShantou University Medical CollegeShantou University Medical CollegeAdministration of mannitol with high dose could induce extensive isometric renal proximal tubular vacuolization and acute renal failure in clinic. We previously demonstrated that mannitol-induced human kidney tubular epithelial cell (HK-2) injury. The objective of our present work was to further study the cytotoxicity of mannitol in HK-2 cells and its potential mechanism. Cell viability was assessed by an MTT method. Cell morphological changes were observed. Furthermore, levels of malondialdehyde (MDA) and glutathione (GSH) were measured. Flow cytometry was performed to determine cell apoptosis by using Annexin V-FITC and PI. In addition, the F-actin of cells was labeled by FITC-Phalloidin for observation of cytoskeleton. The MTT assay displayed that the cell viability decreased significantly in a dose- and time-dependent manner. The morphological changes were observed, including cell membrane rapture and cell detachment. The GSH concentration in HK-2 cells decreased dramatically in mannitol treatment group, while MDA content increased significantly. The results of flow cytometry indicated that apoptotic percentages of HK-2 cells increased in 250 mmol/L mannitol treatment group. After treatment with 250 mmol/L mannitol for 48 h, HK-2 cells showed disorganization of cytoskeleton and even exhibited a totally destroyed cytoskeleton. Therefore, high dose of mannitol has a toxic effect on renal tubular epithelial cells, which might be attributed to oxidative stress, destroyed cellular cytoskeleton and subsequent cell apoptosis.http://dx.doi.org/10.1080/0886022X.2017.1419973Cell apoptosiscytoskeletoncytotoxicityHK-2 cellmannitoloxidative stress
collection DOAJ
language English
format Article
sources DOAJ
author Jinwan Shi
Jiuzhan Qian
Hui Li
Hongjun Luo
Wenhong Luo
Zhexuan Lin
spellingShingle Jinwan Shi
Jiuzhan Qian
Hui Li
Hongjun Luo
Wenhong Luo
Zhexuan Lin
Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
Renal Failure
Cell apoptosis
cytoskeleton
cytotoxicity
HK-2 cell
mannitol
oxidative stress
author_facet Jinwan Shi
Jiuzhan Qian
Hui Li
Hongjun Luo
Wenhong Luo
Zhexuan Lin
author_sort Jinwan Shi
title Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
title_short Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
title_full Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
title_fullStr Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
title_full_unstemmed Renal tubular epithelial cells injury induced by mannitol and its potential mechanism
title_sort renal tubular epithelial cells injury induced by mannitol and its potential mechanism
publisher Taylor & Francis Group
series Renal Failure
issn 0886-022X
1525-6049
publishDate 2018-10-01
description Administration of mannitol with high dose could induce extensive isometric renal proximal tubular vacuolization and acute renal failure in clinic. We previously demonstrated that mannitol-induced human kidney tubular epithelial cell (HK-2) injury. The objective of our present work was to further study the cytotoxicity of mannitol in HK-2 cells and its potential mechanism. Cell viability was assessed by an MTT method. Cell morphological changes were observed. Furthermore, levels of malondialdehyde (MDA) and glutathione (GSH) were measured. Flow cytometry was performed to determine cell apoptosis by using Annexin V-FITC and PI. In addition, the F-actin of cells was labeled by FITC-Phalloidin for observation of cytoskeleton. The MTT assay displayed that the cell viability decreased significantly in a dose- and time-dependent manner. The morphological changes were observed, including cell membrane rapture and cell detachment. The GSH concentration in HK-2 cells decreased dramatically in mannitol treatment group, while MDA content increased significantly. The results of flow cytometry indicated that apoptotic percentages of HK-2 cells increased in 250 mmol/L mannitol treatment group. After treatment with 250 mmol/L mannitol for 48 h, HK-2 cells showed disorganization of cytoskeleton and even exhibited a totally destroyed cytoskeleton. Therefore, high dose of mannitol has a toxic effect on renal tubular epithelial cells, which might be attributed to oxidative stress, destroyed cellular cytoskeleton and subsequent cell apoptosis.
topic Cell apoptosis
cytoskeleton
cytotoxicity
HK-2 cell
mannitol
oxidative stress
url http://dx.doi.org/10.1080/0886022X.2017.1419973
work_keys_str_mv AT jinwanshi renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
AT jiuzhanqian renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
AT huili renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
AT hongjunluo renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
AT wenhongluo renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
AT zhexuanlin renaltubularepithelialcellsinjuryinducedbymannitolanditspotentialmechanism
_version_ 1724941387689885696