Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues

Objective To observe hypoxic injuries in 7 cell lines derived from different human tissues to identify a cell model that is sensitive to acute hypoxic injury. Methods Seven cell lines derived from different human tissues, namely CCD 841 CoN, GES-1, HUVEC, HEB, HEK-293, HBE and L02, were exposed to d...

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Main Authors: FENG Lan, CHEN Dewei, GAO Wenxiang
Format: Article
Language:zho
Published: Editorial Office of Journal of Third Military Medical University 2019-03-01
Series:Di-san junyi daxue xuebao
Subjects:
Online Access:http://aammt.tmmu.edu.cn/Upload/rhtml/201808173.htm
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spelling doaj-beaed94bfbc942dba2aa233ae7d35b3d2021-06-07T07:56:29ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042019-03-0141657057410.16016/j.1000-5404.201808173Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues FENG Lan0FENG Lan1CHEN Dewei2CHEN Dewei3GAO Wenxiang4 Institute of Medicine and Equipment for High Altitude Region, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Key Laboratory of High Altitude Medicine, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Key Laboratory of High Altitude Medicine, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaDepartment of Pathophysiology, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Institute of Medicine and Equipment for High Altitude Region, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaObjective To observe hypoxic injuries in 7 cell lines derived from different human tissues to identify a cell model that is sensitive to acute hypoxic injury. Methods Seven cell lines derived from different human tissues, namely CCD 841 CoN, GES-1, HUVEC, HEB, HEK-293, HBE and L02, were exposed to different oxygen concentrations (1% and 5%) for 24 h using a hypoxia workstation. CCK-8 and LDH kits were used to assess the cell proliferation and cell injuries. The cells were then treated with rhodiola (0.625 μmol/L, 2.5 mmol/L) and 3-n-butylphthalide (1, 2 μmol/L) at the start of hypoxic exposure to assess the protective effects of the drugs against hypoxic cell injuries. Results Compared with the cells cultured in normal oxygen concentration (21%), HEB cells exposed to 1% O2 for 24 h showed significantly lowered proliferative activity (P < 0.05). Hypoxic exposure to 5% or 1% O2 for 24 h significantly increased LDH activity in the culture supernatant of HEB, HUVEC, HBE, HEK-293, CCD 841 CoN, GES-1 and L02 cells (P < 0.05 or 0.01). In HEB cells cultured under hypoxic conditions, treatment with rhodiola and 3-n-butylphthalide significantly promoted the cell proliferation and improved the cell viability (P < 0.05), and obviously reversed hypoxia-induced increase in LDH activity (P < 0.05 or P < 0.01). Conclusion Among the 7 cell lines tested in this study, HEB cells are the most sensitive to hypoxia and can serve as an ideal model for studying drug interventions of hypoxic cell injuries. http://aammt.tmmu.edu.cn/Upload/rhtml/201808173.htmhypoxiatissue cellscell proliferationlactic dehydrogenase
collection DOAJ
language zho
format Article
sources DOAJ
author FENG Lan
FENG Lan
CHEN Dewei
CHEN Dewei
GAO Wenxiang
spellingShingle FENG Lan
FENG Lan
CHEN Dewei
CHEN Dewei
GAO Wenxiang
Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
Di-san junyi daxue xuebao
hypoxia
tissue cells
cell proliferation
lactic dehydrogenase
author_facet FENG Lan
FENG Lan
CHEN Dewei
CHEN Dewei
GAO Wenxiang
author_sort FENG Lan
title Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
title_short Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
title_full Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
title_fullStr Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
title_full_unstemmed Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
title_sort evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
publisher Editorial Office of Journal of Third Military Medical University
series Di-san junyi daxue xuebao
issn 1000-5404
publishDate 2019-03-01
description Objective To observe hypoxic injuries in 7 cell lines derived from different human tissues to identify a cell model that is sensitive to acute hypoxic injury. Methods Seven cell lines derived from different human tissues, namely CCD 841 CoN, GES-1, HUVEC, HEB, HEK-293, HBE and L02, were exposed to different oxygen concentrations (1% and 5%) for 24 h using a hypoxia workstation. CCK-8 and LDH kits were used to assess the cell proliferation and cell injuries. The cells were then treated with rhodiola (0.625 μmol/L, 2.5 mmol/L) and 3-n-butylphthalide (1, 2 μmol/L) at the start of hypoxic exposure to assess the protective effects of the drugs against hypoxic cell injuries. Results Compared with the cells cultured in normal oxygen concentration (21%), HEB cells exposed to 1% O2 for 24 h showed significantly lowered proliferative activity (P < 0.05). Hypoxic exposure to 5% or 1% O2 for 24 h significantly increased LDH activity in the culture supernatant of HEB, HUVEC, HBE, HEK-293, CCD 841 CoN, GES-1 and L02 cells (P < 0.05 or 0.01). In HEB cells cultured under hypoxic conditions, treatment with rhodiola and 3-n-butylphthalide significantly promoted the cell proliferation and improved the cell viability (P < 0.05), and obviously reversed hypoxia-induced increase in LDH activity (P < 0.05 or P < 0.01). Conclusion Among the 7 cell lines tested in this study, HEB cells are the most sensitive to hypoxia and can serve as an ideal model for studying drug interventions of hypoxic cell injuries.
topic hypoxia
tissue cells
cell proliferation
lactic dehydrogenase
url http://aammt.tmmu.edu.cn/Upload/rhtml/201808173.htm
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