| Summary: | In
this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant
activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging capacity, 2.2′‐azinobis
(3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging
capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic
and flavonoid contents of extracts were determined by Folin-Ciocalteu and
aluminum chloride methods, respectively. Also, anti-inflammatory activity of
these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate
extract of capitula of T. cilicicum
(TCCEA) showed the highest antioxidant activity with IC50 values of
22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same
time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g
extract). The highest total phenolic contents have been detected in TCCEA and
ethyl acetate extract of leaves of T.
cilicicum (TCLEA) with value of 174.1 and 175.6 mg GAE/g extract,
respectively. Similarly, the highest total flavonoid contents have been
detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract,
respectively. TCCEA exhibited strong anti-inflammatory activity with IC50
value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL).
These results demonstrate that TCCEA has a significant antioxidant and
anti-inflammatory activity. Also, the results show that TCCEA is a good
candidate for further bioactivity-guided fractionation in the search for new
active anti-inflammatory and antioxidant compounds.
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