Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil
The commercial release of genetically modified organisms (GMO) requires a prior environmental and human/animal health risk assessment. In Brazil, the National Biotechnology Technical Commission (CTNBio) requires a survey of the area of natural occurrence of wild relatives of the GMO in the Brazilian...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-06-01
|
Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00598/full |
id |
doaj-be508b7309d84236a9fc93c3957082e8 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eduardo Andrade Bressan Igor Araújo Santos de Carvalho Maria Teresa Mendes Ribeiro Borges Monalisa Sampaio Carneiro Edson Ferreira da Silva Rodrigo Gazaffi Regina Tomoko Shirasuna Vinícius Abreu Rafael V. Popin Antonio Figueira Giancarlo Conde Xavier Oliveira |
spellingShingle |
Eduardo Andrade Bressan Igor Araújo Santos de Carvalho Maria Teresa Mendes Ribeiro Borges Monalisa Sampaio Carneiro Edson Ferreira da Silva Rodrigo Gazaffi Regina Tomoko Shirasuna Vinícius Abreu Rafael V. Popin Antonio Figueira Giancarlo Conde Xavier Oliveira Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil Frontiers in Bioengineering and Biotechnology interspecific hybrids natural hybridization Saccharum asperum Saccharum angustifolium Saccharum villosum Saccharum × officinarum |
author_facet |
Eduardo Andrade Bressan Igor Araújo Santos de Carvalho Maria Teresa Mendes Ribeiro Borges Monalisa Sampaio Carneiro Edson Ferreira da Silva Rodrigo Gazaffi Regina Tomoko Shirasuna Vinícius Abreu Rafael V. Popin Antonio Figueira Giancarlo Conde Xavier Oliveira |
author_sort |
Eduardo Andrade Bressan |
title |
Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil |
title_short |
Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil |
title_full |
Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil |
title_fullStr |
Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil |
title_full_unstemmed |
Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil |
title_sort |
assessment of gene flow to wild relatives and nutritional composition of sugarcane in brazil |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2020-06-01 |
description |
The commercial release of genetically modified organisms (GMO) requires a prior environmental and human/animal health risk assessment. In Brazil, the National Biotechnology Technical Commission (CTNBio) requires a survey of the area of natural occurrence of wild relatives of the GMO in the Brazilian ecosystems to evaluate the possibility of introgressive hybridization between sexually compatible species. Modern sugarcane cultivars, the focus of this study, derive from a series of hybridization and backcrossing events among Saccharum species. The so-called “Saccharum broad sense” group includes around 40 species from a few genera, including Erianthus, found in various tropical regions, particularly South-Eastern Asia. In Brazil, three native species, originally considered to belong to Erianthus, were reclassified as S. angustifolium (Nees) Trin., S. asperum (Nees) Steud., and S. villosum Steud., based on inflorescence morphology. Thus, we have investigated the potential occurrence of gene flow among the Brazilian Saccharum native species and commercial hybrids as a requisite for GMO commercial release. A comprehensive survey was carried out to map the occurrence of the three native Saccharum species in Brazil, concluding that they are sympatric with sugarcane cultivation only from around 14°S southwards, which precludes most Northeastern sugarcane-producing states from undergoing introgression. Based on phenology, we concluded that the Brazilian Saccharum species are unable to outcross naturally with commercial sugarcane since the overlap between the flowering periods of sugarcane and the native species is limited. A phylogenomic reconstruction based on the full plastid genome sequence showed that the three native Saccharum species are the taxa closest to sugarcane in Brazil, being closer than introduced Erianthus or Miscanthus. A 2-year study on eight nutritional composition traits of the 20 main sugarcane cultivars cultivated in Brazil was carried out in six environments. The minimum and maximum values obtained were, in percent: moisture (62.6–82.5); sucrose (9.65–21.76); crude fiber (8.06–21.03); FDN (7.20–20.68); FDA (4.55–16.90); lipids (0.06–1.59); ash (0.08–2.67); and crude protein (0.18–1.18). Besides a considerable amount of genetic variation and plastic responses, many instances of genotype-by-environment interaction were detected. |
topic |
interspecific hybrids natural hybridization Saccharum asperum Saccharum angustifolium Saccharum villosum Saccharum × officinarum |
url |
https://www.frontiersin.org/article/10.3389/fbioe.2020.00598/full |
work_keys_str_mv |
AT eduardoandradebressan assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT igoraraujosantosdecarvalho assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT mariateresamendesribeiroborges assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT monalisasampaiocarneiro assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT edsonferreiradasilva assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT rodrigogazaffi assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT reginatomokoshirasuna assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT viniciusabreu assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT rafaelvpopin assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT antoniofigueira assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil AT giancarlocondexavieroliveira assessmentofgeneflowtowildrelativesandnutritionalcompositionofsugarcaneinbrazil |
_version_ |
1724639078513639424 |
spelling |
doaj-be508b7309d84236a9fc93c3957082e82020-11-25T03:15:31ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-06-01810.3389/fbioe.2020.00598532373Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in BrazilEduardo Andrade Bressan0Igor Araújo Santos de Carvalho1Maria Teresa Mendes Ribeiro Borges2Monalisa Sampaio Carneiro3Edson Ferreira da Silva4Rodrigo Gazaffi5Regina Tomoko Shirasuna6Vinícius Abreu7Rafael V. Popin8Antonio Figueira9Giancarlo Conde Xavier Oliveira10Evolution Laboratory, Department of Genetics, “Luiz de Queiroz” Agricultural College, University of São Paulo, Piracicaba, BrazilEvolution Laboratory, Department of Genetics, “Luiz de Queiroz” Agricultural College, University of São Paulo, Piracicaba, BrazilTechnological Analysis and Simulation Laboratory, Department of Agroindustrial Technology and Rural Socioeconomics, Center of Agricultural Sciences, Federal University of São Carlos, Araras, BrazilPlant Biotechnology Laboratory, Department of Biotechnology, Vegetal and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos, Araras, BrazilPlant Breeding Laboratory, Biology Department, Federal Rural University of Pernambuco, Recife, BrazilDepartment of Biotechnology, Vegetal and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos, Araras, BrazilHerbarium Curatorship Research Nucleus, Vascular Plants Research Center, Institute of Botany, São Paulo, BrazilLaboratory of Cell and Molecular Biology, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, BrazilLaboratory of Cell and Molecular Biology, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, BrazilPlant Breeding Laboratory, Center of Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, BrazilEvolution Laboratory, Department of Genetics, “Luiz de Queiroz” Agricultural College, University of São Paulo, Piracicaba, BrazilThe commercial release of genetically modified organisms (GMO) requires a prior environmental and human/animal health risk assessment. In Brazil, the National Biotechnology Technical Commission (CTNBio) requires a survey of the area of natural occurrence of wild relatives of the GMO in the Brazilian ecosystems to evaluate the possibility of introgressive hybridization between sexually compatible species. Modern sugarcane cultivars, the focus of this study, derive from a series of hybridization and backcrossing events among Saccharum species. The so-called “Saccharum broad sense” group includes around 40 species from a few genera, including Erianthus, found in various tropical regions, particularly South-Eastern Asia. In Brazil, three native species, originally considered to belong to Erianthus, were reclassified as S. angustifolium (Nees) Trin., S. asperum (Nees) Steud., and S. villosum Steud., based on inflorescence morphology. Thus, we have investigated the potential occurrence of gene flow among the Brazilian Saccharum native species and commercial hybrids as a requisite for GMO commercial release. A comprehensive survey was carried out to map the occurrence of the three native Saccharum species in Brazil, concluding that they are sympatric with sugarcane cultivation only from around 14°S southwards, which precludes most Northeastern sugarcane-producing states from undergoing introgression. Based on phenology, we concluded that the Brazilian Saccharum species are unable to outcross naturally with commercial sugarcane since the overlap between the flowering periods of sugarcane and the native species is limited. A phylogenomic reconstruction based on the full plastid genome sequence showed that the three native Saccharum species are the taxa closest to sugarcane in Brazil, being closer than introduced Erianthus or Miscanthus. A 2-year study on eight nutritional composition traits of the 20 main sugarcane cultivars cultivated in Brazil was carried out in six environments. The minimum and maximum values obtained were, in percent: moisture (62.6–82.5); sucrose (9.65–21.76); crude fiber (8.06–21.03); FDN (7.20–20.68); FDA (4.55–16.90); lipids (0.06–1.59); ash (0.08–2.67); and crude protein (0.18–1.18). Besides a considerable amount of genetic variation and plastic responses, many instances of genotype-by-environment interaction were detected.https://www.frontiersin.org/article/10.3389/fbioe.2020.00598/fullinterspecific hybridsnatural hybridizationSaccharum asperumSaccharum angustifoliumSaccharum villosumSaccharum × officinarum |