Engineered ribosomes with tethered subunits for expanding biological function

Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.

Bibliographic Details
Main Authors: Erik D. Carlson, Anne E. d’Aquino, Do Soon Kim, Emily M. Fulk, Kim Hoang, Teresa Szal, Alexander S. Mankin, Michael C. Jewett
Format: Article
Language:English
Published: Nature Publishing Group 2019-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-11427-y
Description
Summary:Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.
ISSN:2041-1723