Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan

Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan

This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterho...

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Main Authors: A.E. El Tigani-Asil, S.M. EL Sanousi, M.A. Aljameel, H. El Beir, A. Adam, M.M. Abdallatif, M.E. Hamid
Format: Article
Language:English
Published: Tripoli University 2014-02-01
Series:Open Veterinary Journal
Subjects:
Acid fast bacteria
Bovine farcy
Nontuberculous mycobacteria
Pyogenic infection
Online Access:http://www.openveterinaryjournal.com/2014/Volume%204%20(1)/OVJ-157-11-13%20E.A.%20EL%20Tigani-Asil%20et%20al.pdf
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spelling doaj-be0b886463e54cb08b53a1e981adc7e32021-10-02T06:53:44ZengTripoli UniversityOpen Veterinary Journal2218-60502218-60502014-02-01411619Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, SudanA.E. El Tigani-Asil0S.M. EL Sanousi1M.A. Aljameel2H. El Beir3A. Adam4M.M. Abdallatif5M.E. Hamid6Departments of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Qassim University, Saudi ArabiaDepartments of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, SudanDepartment of Pathology, Veterinary Research laboratory - Nyala, National Veterinary Research Corporation, SudanDepartment of Tuberculosis, Institute of Tropical Diseases, Ministry of Science and Technology, SudanDepartment of Pathology, Faculty of Veterinary Science, University of Nyala, SudanDepartment of Microbiology, Faculty of Science, North Border University, Saudi ArabiaDepartment of Clinical Microbiology and Parasitology, College of Medicine, King Khalid University, Saudi ArabiaThis study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated. http://www.openveterinaryjournal.com/2014/Volume%204%20(1)/OVJ-157-11-13%20E.A.%20EL%20Tigani-Asil%20et%20al.pdfAcid fast bacteriaBovine farcyNontuberculous mycobacteriaPyogenic infection
collection DOAJ
language English
format Article
sources DOAJ
author A.E. El Tigani-Asil
S.M. EL Sanousi
M.A. Aljameel
H. El Beir
A. Adam
M.M. Abdallatif
M.E. Hamid
spellingShingle A.E. El Tigani-Asil
S.M. EL Sanousi
M.A. Aljameel
H. El Beir
A. Adam
M.M. Abdallatif
M.E. Hamid
Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
Open Veterinary Journal
Acid fast bacteria
Bovine farcy
Nontuberculous mycobacteria
Pyogenic infection
author_facet A.E. El Tigani-Asil
S.M. EL Sanousi
M.A. Aljameel
H. El Beir
A. Adam
M.M. Abdallatif
M.E. Hamid
author_sort A.E. El Tigani-Asil
title Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_short Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_full Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_fullStr Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_full_unstemmed Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan
title_sort molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in south darfur state and alsabalouga slaughterhouse at omdurman area, sudan
publisher Tripoli University
series Open Veterinary Journal
issn 2218-6050
2218-6050
publishDate 2014-02-01
description This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.
topic Acid fast bacteria
Bovine farcy
Nontuberculous mycobacteria
Pyogenic infection
url http://www.openveterinaryjournal.com/2014/Volume%204%20(1)/OVJ-157-11-13%20E.A.%20EL%20Tigani-Asil%20et%20al.pdf
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