Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis

Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis

Abstract Background Lactococcus lactis is a food grade probiotics and widely used to express heterologous proteins. Generally, target genes are knocked into the L. lactis genome through double-crossover recombination to express heterologous proteins stably. However, creating marker-less heterologous...

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Bibliographic Details
Main Authors: Yifei Lu, Hongxiang Yan, Jiezhong Deng, Zhigang Huang, Xurui Jin, Yanlan Yu, Qiwen Hu, Fuquan Hu, Jing Wang
Format: Article
Language:English
Published: BMC 2017-09-01
Series:Microbial Cell Factories
Subjects:
Lactococcus lactis
NZ9000
Knocked-in heterologous gene
Knock-in reporter system
lacZ
Online Access:http://link.springer.com/article/10.1186/s12934-017-0770-1

Internet

http://link.springer.com/article/10.1186/s12934-017-0770-1

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