Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis

Evaluation of usefulness of single-strand conformation polymorphism method for rapid detection of rifampicin-resistant mycobacterium tuberculosis

The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifamp...

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Bibliographic Details
Main Authors: Tešić Jelena, Lepšanović Zorica, Mirović Veljko
Format: Article
Language:English
Published: Military Health Department, Ministry of Defance, Serbia 2004-01-01
Series:Vojnosanitetski Pregled
Subjects:
polymorphism
single-standed conformational
mycobacterum tuberculosis
rifampin
drug resistance
microbial
Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500402169T.pdf
Description
Summary:The aim was to evaluate the Single-Strand Conformation Polymorphism method as a potential tool for rapid detection of rifampicin-resistant strains by the use of 39 rifampicin-resistant Mycobacterium tuberculosis strains isolated in Serbia. SSCP analysis on acrylamide gel detected 56.4% of the rifampicin-resistant M. tuberculosis strains and showed the inability to detect one of the most frequent mutations, TCG®TTG mutation in codon 531 of the rpoB gene, which was shown by automated sequencing.
ISSN:0042-8450

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