Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

Summary: We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage th...

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Bibliographic Details
Main Authors: Aymeric Chorlay, Alexandre Santinho, Abdou Rachid Thiam
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301039

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