Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

• Main Authors: María F. Pomponio, Cintia Acuña, Vivien Pentreath, Diego L. Lauenstein, Susana M. Poltri, Susana Torales
• Format: Article
• Language: English
• Published: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria 2015-08-01
• Series: Forest Systems
• Subjects: genetic variation; functional markers; microsatellites; prosopis
• Online Access: http://revistas.inia.es/index.php/fs/article/view/7188/2460

Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P.alba, P. denudans, P. hassleriP. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.