Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
Throughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24743-z |
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doaj-bd881c2a13fb42f1a6bfafcd640b11a42021-07-25T11:39:12ZengNature Publishing GroupNature Communications2041-17232021-07-0112111210.1038/s41467-021-24743-zMassively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturationNathan J. VanDusen0Julianna Y. Lee1Weiliang Gu2Catalina E. Butler3Isha Sethi4Yanjiang Zheng5Justin S. King6Pingzhu Zhou7Shengbao Suo8Yuxuan Guo9Qing Ma10Guo-Cheng Yuan11William William Pu12Department of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Pediatric Oncology, Dana-Farber Cancer InstituteDepartment of Cardiology, Boston Children’s HospitalDepartment of Cardiology, Boston Children’s HospitalDepartment of Genetics and Genomic Sciences and Institute for Personalized Medicine, Icahn School of Medicine at Mount SinaiDepartment of Cardiology, Boston Children’s HospitalThroughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40.https://doi.org/10.1038/s41467-021-24743-z |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu |
| spellingShingle |
Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation Nature Communications |
| author_facet |
Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu |
| author_sort |
Nathan J. VanDusen |
| title |
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation |
| title_short |
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation |
| title_full |
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation |
| title_fullStr |
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation |
| title_full_unstemmed |
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation |
| title_sort |
massively parallel in vivo crispr screening identifies rnf20/40 as epigenetic regulators of cardiomyocyte maturation |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-07-01 |
| description |
Throughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40. |
| url |
https://doi.org/10.1038/s41467-021-24743-z |
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