A Novel Quantum Dots–Based Point of Care Test for Syphilis
<p>Abstract</p> <p>One-step lateral flow test is recommended as the first line screening of <it>syphilis</it> for primary healthcare settings in developing countries. However, it generally shows low sensitivity. We describe here the development of a novel fluorescent PO...
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2010-01-01
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| Series: | Nanoscale Research Letters |
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| Online Access: | http://dx.doi.org/10.1007/s11671-010-9578-1 |
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doaj-bd71419b4bb244c789cbcf16362f2e262020-11-24T20:56:25ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2010-01-0155875881A Novel Quantum Dots–Based Point of Care Test for SyphilisLi DingYang HaoHe RongGuo QinWang KanZhang XueqingHuang PengCui Daxiang<p>Abstract</p> <p>One-step lateral flow test is recommended as the first line screening of <it>syphilis</it> for primary healthcare settings in developing countries. However, it generally shows low sensitivity. We describe here the development of a novel fluorescent POC (Point Of Care) test method to be used for screening for syphilis. The method was designed to combine the rapidness of lateral flow test and sensitiveness of fluorescent method. 50 <it>syphilis</it>-positive specimens and 50 healthy specimens conformed by <it>Treponema pallidum</it> particle agglutination (TPPA) were tested with Quantum Dot-labeled and colloidal gold-labeled lateral flow test strips, respectively. The results showed that both sensitivity and specificity of the quantum dots–based method reached up to 100% (95% confidence interval [CI], 91–100%), while those of the colloidal gold-based method were 82% (95% CI, 68–91%) and 100% (95% CI, 91–100%), respectively. In addition, the naked-eye detection limit of quantum dot–based method could achieve 2 ng/ml of anti-TP47 polyclonal antibodies purified by affinity chromatography with TP47 antigen, which was tenfold higher than that of colloidal gold–based method. In conclusion, the quantum dots were found to be suitable for labels of lateral flow test strip. Its ease of use, sensitiveness and low cost make it well-suited for population-based on-the-site syphilis screening.</p> http://dx.doi.org/10.1007/s11671-010-9578-1SyphilisLateral flowDiagnosticsQuantum dotsNanomaterial |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Li Ding Yang Hao He Rong Guo Qin Wang Kan Zhang Xueqing Huang Peng Cui Daxiang |
| spellingShingle |
Li Ding Yang Hao He Rong Guo Qin Wang Kan Zhang Xueqing Huang Peng Cui Daxiang A Novel Quantum Dots–Based Point of Care Test for Syphilis Nanoscale Research Letters Syphilis Lateral flow Diagnostics Quantum dots Nanomaterial |
| author_facet |
Li Ding Yang Hao He Rong Guo Qin Wang Kan Zhang Xueqing Huang Peng Cui Daxiang |
| author_sort |
Li Ding |
| title |
A Novel Quantum Dots–Based Point of Care Test for Syphilis |
| title_short |
A Novel Quantum Dots–Based Point of Care Test for Syphilis |
| title_full |
A Novel Quantum Dots–Based Point of Care Test for Syphilis |
| title_fullStr |
A Novel Quantum Dots–Based Point of Care Test for Syphilis |
| title_full_unstemmed |
A Novel Quantum Dots–Based Point of Care Test for Syphilis |
| title_sort |
novel quantum dots–based point of care test for syphilis |
| publisher |
SpringerOpen |
| series |
Nanoscale Research Letters |
| issn |
1931-7573 1556-276X |
| publishDate |
2010-01-01 |
| description |
<p>Abstract</p> <p>One-step lateral flow test is recommended as the first line screening of <it>syphilis</it> for primary healthcare settings in developing countries. However, it generally shows low sensitivity. We describe here the development of a novel fluorescent POC (Point Of Care) test method to be used for screening for syphilis. The method was designed to combine the rapidness of lateral flow test and sensitiveness of fluorescent method. 50 <it>syphilis</it>-positive specimens and 50 healthy specimens conformed by <it>Treponema pallidum</it> particle agglutination (TPPA) were tested with Quantum Dot-labeled and colloidal gold-labeled lateral flow test strips, respectively. The results showed that both sensitivity and specificity of the quantum dots–based method reached up to 100% (95% confidence interval [CI], 91–100%), while those of the colloidal gold-based method were 82% (95% CI, 68–91%) and 100% (95% CI, 91–100%), respectively. In addition, the naked-eye detection limit of quantum dot–based method could achieve 2 ng/ml of anti-TP47 polyclonal antibodies purified by affinity chromatography with TP47 antigen, which was tenfold higher than that of colloidal gold–based method. In conclusion, the quantum dots were found to be suitable for labels of lateral flow test strip. Its ease of use, sensitiveness and low cost make it well-suited for population-based on-the-site syphilis screening.</p> |
| topic |
Syphilis Lateral flow Diagnostics Quantum dots Nanomaterial |
| url |
http://dx.doi.org/10.1007/s11671-010-9578-1 |
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