Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.

Bibliographic Details
Main Authors:	Youngbin Lim, So Young Bak, Keewon Sung, Euihwan Jeong, Seung Hwan Lee, Jin-Soo Kim, Sangsu Bae, Seong Keun Kim
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms13350

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spelling	doaj-bd61a1e9e4df45b785666f33100ca9fe2021-05-11T11:20:48ZengNature Publishing GroupNature Communications2041-17232016-11-01711810.1038/ncomms13350Structural roles of guide RNAs in the nuclease activity of Cas9 endonucleaseYoungbin Lim0So Young Bak1Keewon Sung2Euihwan Jeong3Seung Hwan Lee4Jin-Soo Kim5Sangsu Bae6Seong Keun Kim7Department of Biophysics and Chemical Biology, Seoul National UniversityDepartment of Chemistry, Seoul National UniversityDepartment of Chemistry, Seoul National UniversityDepartment of Chemistry, Seoul National UniversityCenter for Genome Engineering, Institute for Basic ScienceDepartment of Chemistry, Seoul National UniversityDepartment of Chemistry, Seoul National UniversityDepartment of Biophysics and Chemical Biology, Seoul National UniversityIn bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.https://doi.org/10.1038/ncomms13350
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim
spellingShingle	Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease Nature Communications
author_facet	Youngbin Lim So Young Bak Keewon Sung Euihwan Jeong Seung Hwan Lee Jin-Soo Kim Sangsu Bae Seong Keun Kim
author_sort	Youngbin Lim
title	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_short	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_full	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_fullStr	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_full_unstemmed	Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
title_sort	structural roles of guide rnas in the nuclease activity of cas9 endonuclease
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-11-01
description	In bacteria, CRISPR-Cas9 identifies and cleaves the target DNA with the assistance of a tracrRNA and a crRNA. Here the authors use single-molecule spectroscopy to investigate conformational changes and show that the tracrRNA keeps Cas9 in a functionally active form.
url	https://doi.org/10.1038/ncomms13350
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Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease

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