Exploring the whole standard operating procedure for phage therapy in clinical practice
Abstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage th...
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doaj-bd5be8e84a8741639caf1363232daea32020-11-25T04:05:21ZengBMCJournal of Translational Medicine1479-58762019-11-011711710.1186/s12967-019-2120-zExploring the whole standard operating procedure for phage therapy in clinical practiceZelin Cui0Xiaokui Guo1Tingting Feng2Li Li3Department of Laboratory Medicine, Shanghai General HospitalDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of MedicineDepartment of Clinical Pharmacy, Shanghai General HospitalDepartment of Laboratory Medicine, Shanghai General HospitalAbstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage therapy is not as effective as expected. In general, establishment of a standard operating procedure will ensure the success of phage therapy. In this paper, the whole operating procedure for phage therapy in clinical practice is explored and analyzed to comprehensively understand the success of using phage for the treatment of bacterial infectious disease in the future. The procedure includes the following: enrollment of patients for phage therapy; establishment of phage libraries; pathogenic bacterial isolation and identification; screening for effective phages against pathogenic bacteria; phage formulation preparation; phage preparation administration strategy and route; monitoring the efficacy of phage therapy; and detection of the emergence of phage-resistant strains. Finally, we outline the whole standard operating procedure for phage therapy in clinical practice. It is believed that phage therapy will be used successfully, especially in personalized medicine for the treatment of bacterial infectious diseases. Hopefully, this procedure will provide support for the entry of phage therapy into the clinic as soon as possible.http://link.springer.com/article/10.1186/s12967-019-2120-zPhage therapyClinical practiceStreamlineStandard operating proceduresAntibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zelin Cui Xiaokui Guo Tingting Feng Li Li |
spellingShingle |
Zelin Cui Xiaokui Guo Tingting Feng Li Li Exploring the whole standard operating procedure for phage therapy in clinical practice Journal of Translational Medicine Phage therapy Clinical practice Streamline Standard operating procedures Antibiotic resistance |
author_facet |
Zelin Cui Xiaokui Guo Tingting Feng Li Li |
author_sort |
Zelin Cui |
title |
Exploring the whole standard operating procedure for phage therapy in clinical practice |
title_short |
Exploring the whole standard operating procedure for phage therapy in clinical practice |
title_full |
Exploring the whole standard operating procedure for phage therapy in clinical practice |
title_fullStr |
Exploring the whole standard operating procedure for phage therapy in clinical practice |
title_full_unstemmed |
Exploring the whole standard operating procedure for phage therapy in clinical practice |
title_sort |
exploring the whole standard operating procedure for phage therapy in clinical practice |
publisher |
BMC |
series |
Journal of Translational Medicine |
issn |
1479-5876 |
publishDate |
2019-11-01 |
description |
Abstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage therapy is not as effective as expected. In general, establishment of a standard operating procedure will ensure the success of phage therapy. In this paper, the whole operating procedure for phage therapy in clinical practice is explored and analyzed to comprehensively understand the success of using phage for the treatment of bacterial infectious disease in the future. The procedure includes the following: enrollment of patients for phage therapy; establishment of phage libraries; pathogenic bacterial isolation and identification; screening for effective phages against pathogenic bacteria; phage formulation preparation; phage preparation administration strategy and route; monitoring the efficacy of phage therapy; and detection of the emergence of phage-resistant strains. Finally, we outline the whole standard operating procedure for phage therapy in clinical practice. It is believed that phage therapy will be used successfully, especially in personalized medicine for the treatment of bacterial infectious diseases. Hopefully, this procedure will provide support for the entry of phage therapy into the clinic as soon as possible. |
topic |
Phage therapy Clinical practice Streamline Standard operating procedures Antibiotic resistance |
url |
http://link.springer.com/article/10.1186/s12967-019-2120-z |
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