Exploring the whole standard operating procedure for phage therapy in clinical practice

Abstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage th...

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Main Authors: Zelin Cui, Xiaokui Guo, Tingting Feng, Li Li
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Journal of Translational Medicine
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12967-019-2120-z
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spelling doaj-bd5be8e84a8741639caf1363232daea32020-11-25T04:05:21ZengBMCJournal of Translational Medicine1479-58762019-11-011711710.1186/s12967-019-2120-zExploring the whole standard operating procedure for phage therapy in clinical practiceZelin Cui0Xiaokui Guo1Tingting Feng2Li Li3Department of Laboratory Medicine, Shanghai General HospitalDepartment of Immunology and Microbiology, Shanghai Jiao Tong University School of MedicineDepartment of Clinical Pharmacy, Shanghai General HospitalDepartment of Laboratory Medicine, Shanghai General HospitalAbstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage therapy is not as effective as expected. In general, establishment of a standard operating procedure will ensure the success of phage therapy. In this paper, the whole operating procedure for phage therapy in clinical practice is explored and analyzed to comprehensively understand the success of using phage for the treatment of bacterial infectious disease in the future. The procedure includes the following: enrollment of patients for phage therapy; establishment of phage libraries; pathogenic bacterial isolation and identification; screening for effective phages against pathogenic bacteria; phage formulation preparation; phage preparation administration strategy and route; monitoring the efficacy of phage therapy; and detection of the emergence of phage-resistant strains. Finally, we outline the whole standard operating procedure for phage therapy in clinical practice. It is believed that phage therapy will be used successfully, especially in personalized medicine for the treatment of bacterial infectious diseases. Hopefully, this procedure will provide support for the entry of phage therapy into the clinic as soon as possible.http://link.springer.com/article/10.1186/s12967-019-2120-zPhage therapyClinical practiceStreamlineStandard operating proceduresAntibiotic resistance
collection DOAJ
language English
format Article
sources DOAJ
author Zelin Cui
Xiaokui Guo
Tingting Feng
Li Li
spellingShingle Zelin Cui
Xiaokui Guo
Tingting Feng
Li Li
Exploring the whole standard operating procedure for phage therapy in clinical practice
Journal of Translational Medicine
Phage therapy
Clinical practice
Streamline
Standard operating procedures
Antibiotic resistance
author_facet Zelin Cui
Xiaokui Guo
Tingting Feng
Li Li
author_sort Zelin Cui
title Exploring the whole standard operating procedure for phage therapy in clinical practice
title_short Exploring the whole standard operating procedure for phage therapy in clinical practice
title_full Exploring the whole standard operating procedure for phage therapy in clinical practice
title_fullStr Exploring the whole standard operating procedure for phage therapy in clinical practice
title_full_unstemmed Exploring the whole standard operating procedure for phage therapy in clinical practice
title_sort exploring the whole standard operating procedure for phage therapy in clinical practice
publisher BMC
series Journal of Translational Medicine
issn 1479-5876
publishDate 2019-11-01
description Abstract We have entered the post-antibiotic era. Phage therapy has recently been given renewed attention because bacteriophages are easily available and can kill bacteria. Many reports have demonstrated successful phage treatment of bacterial infection, whereas some studies have shown that phage therapy is not as effective as expected. In general, establishment of a standard operating procedure will ensure the success of phage therapy. In this paper, the whole operating procedure for phage therapy in clinical practice is explored and analyzed to comprehensively understand the success of using phage for the treatment of bacterial infectious disease in the future. The procedure includes the following: enrollment of patients for phage therapy; establishment of phage libraries; pathogenic bacterial isolation and identification; screening for effective phages against pathogenic bacteria; phage formulation preparation; phage preparation administration strategy and route; monitoring the efficacy of phage therapy; and detection of the emergence of phage-resistant strains. Finally, we outline the whole standard operating procedure for phage therapy in clinical practice. It is believed that phage therapy will be used successfully, especially in personalized medicine for the treatment of bacterial infectious diseases. Hopefully, this procedure will provide support for the entry of phage therapy into the clinic as soon as possible.
topic Phage therapy
Clinical practice
Streamline
Standard operating procedures
Antibiotic resistance
url http://link.springer.com/article/10.1186/s12967-019-2120-z
work_keys_str_mv AT zelincui exploringthewholestandardoperatingprocedureforphagetherapyinclinicalpractice
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AT lili exploringthewholestandardoperatingprocedureforphagetherapyinclinicalpractice
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