The “Dark Side” of Food Stuff Proteomics: The CPLL-Marshals Investigate

• Main Authors: Pier Giorgio Righetti, Elisa Fasoli, Alfonsina D'Amato, Egisto Boschetti
• Format: Article
• Language: English
• Published: MDPI AG 2014-04-01
• Series: Foods
• Subjects: animal foods; plant foods; alcoholic beverages; non-alcoholic beverages; aperitifs; proteomics; combinatorial peptide libraries
• Online Access: http://www.mdpi.com/2304-8158/3/2/217

The present review deals with analysis of the proteome of animal and plant-derived food stuff, as well as of non-alcoholic and alcoholic beverages. The survey is limited to those systems investigated with the help of combinatorial peptide ligand libraries, a most powerful technique allowing access to low- to very-low-abundance proteins, i.e., to those proteins that might characterize univocally a given biological system and, in the case of commercial food preparations, attest their genuineness or adulteration. Among animal foods the analysis of cow’s and donkey’s milk is reported, together with the proteomic composition of egg white and yolk, as well as of honey, considered as a hybrid between floral and animal origin. In terms of plant and fruits, a survey is offered of spinach, artichoke, banana, avocado, mango and lemon proteomics, considered as recalcitrant tissues in that small amounts of proteins are dispersed into a large body of plant polymers and metabolites. As examples of non-alcoholic beverages, ginger ale, coconut milk, a cola drink, almond milk and orgeat syrup are analyzed. Finally, the trace proteome of white and red wines, beer and aperitifs is reported, with the aim of tracing the industrial manipulations and herbal usage prior to their commercialization.