CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibi...
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doaj-bd352654e2b54b73a315422bfae47a472021-05-05T15:37:22ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.32341CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivoGabriel N Aughey0https://orcid.org/0000-0001-5610-9345Alicia Estacio Gomez1Jamie Thomson2Hang Yin3Tony D Southall4https://orcid.org/0000-0002-8645-4198Department of Life Sciences, Imperial College London, London, United KingdomDepartment of Life Sciences, Imperial College London, London, United KingdomDepartment of Life Sciences, Imperial College London, London, United KingdomDepartment of Life Sciences, Imperial College London, London, United KingdomDepartment of Life Sciences, Imperial College London, London, United KingdomDuring development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic lineages are not well defined. Here we show that cell-specific chromatin accessibility data can be produced via ectopic expression of E. coli Dam methylase in vivo, without the requirement for cell-sorting (CATaDa). We have profiled chromatin accessibility in individual cell-types of Drosophila neural and midgut lineages. Functional cell-type-specific enhancers were identified, as well as novel motifs enriched at different stages of development. Finally, we show global changes in the accessibility of chromatin between stem-cells and their differentiated progeny. Our results demonstrate the dynamic nature of chromatin accessibility in somatic tissues during stem cell differentiation and provide a novel approach to understanding gene regulatory mechanisms underlying development.https://elifesciences.org/articles/32341chromatin accessibilityneural developmentdifferentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabriel N Aughey Alicia Estacio Gomez Jamie Thomson Hang Yin Tony D Southall |
spellingShingle |
Gabriel N Aughey Alicia Estacio Gomez Jamie Thomson Hang Yin Tony D Southall CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo eLife chromatin accessibility neural development differentiation |
author_facet |
Gabriel N Aughey Alicia Estacio Gomez Jamie Thomson Hang Yin Tony D Southall |
author_sort |
Gabriel N Aughey |
title |
CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
title_short |
CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
title_full |
CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
title_fullStr |
CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
title_full_unstemmed |
CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
title_sort |
catada reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2018-02-01 |
description |
During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic lineages are not well defined. Here we show that cell-specific chromatin accessibility data can be produced via ectopic expression of E. coli Dam methylase in vivo, without the requirement for cell-sorting (CATaDa). We have profiled chromatin accessibility in individual cell-types of Drosophila neural and midgut lineages. Functional cell-type-specific enhancers were identified, as well as novel motifs enriched at different stages of development. Finally, we show global changes in the accessibility of chromatin between stem-cells and their differentiated progeny. Our results demonstrate the dynamic nature of chromatin accessibility in somatic tissues during stem cell differentiation and provide a novel approach to understanding gene regulatory mechanisms underlying development. |
topic |
chromatin accessibility neural development differentiation |
url |
https://elifesciences.org/articles/32341 |
work_keys_str_mv |
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