Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

While R-loops can alter cell homeostasis, it is unclear what determines their toxicity. Here, the authors, by using Top1 knockdown as a tool to enhance the formation of R-loops at certain genomic sites, reveal and characterize a proportion of R-loops that are more toxic to the cell by causing DNA da...

Full description

Bibliographic Details
Main Authors:	Alexy Promonet, Ismaël Padioleau, Yaqun Liu, Lionel Sanz, Anna Biernacka, Anne-Lyne Schmitz, Magdalena Skrzypczak, Amélie Sarrazin, Clément Mettling, Maga Rowicka, Krzysztof Ginalski, Frédéric Chedin, Chun-Long Chen, Yea-Lih Lin, Philippe Pasero
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-08-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-17858-2

id	doaj-bd1932962b864455ac3b032015f1a0ac
record_format	Article
spelling	doaj-bd1932962b864455ac3b032015f1a0ac2021-08-08T11:38:50ZengNature Publishing GroupNature Communications2041-17232020-08-0111111210.1038/s41467-020-17858-2Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sitesAlexy Promonet0Ismaël Padioleau1Yaqun Liu2Lionel Sanz3Anna Biernacka4Anne-Lyne Schmitz5Magdalena Skrzypczak6Amélie Sarrazin7Clément Mettling8Maga Rowicka9Krzysztof Ginalski10Frédéric Chedin11Chun-Long Chen12Yea-Lih Lin13Philippe Pasero14Institut de Génétique Humaine, CNRS et Université de Montpellier, Equipe labélisée Ligue contre le CancerInstitut de Génétique Humaine, CNRS et Université de Montpellier, Equipe labélisée Ligue contre le CancerInstitut Curie, PSL Research University, CNRS, UMR3244, Sorbonne UniversitéDepartment of Molecular and Cellular Biology, University of CaliforniaLaboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of WarsawInstitut de Génétique Humaine, CNRS et Université de Montpellier, Equipe labélisée Ligue contre le CancerLaboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of WarsawBioCampus Montpellier, CNRS et Université de MontpellierInstitut de Génétique Humaine, CNRS et Université de MontpellierDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch at GalvestonLaboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of WarsawDepartment of Molecular and Cellular Biology, University of CaliforniaInstitut Curie, PSL Research University, CNRS, UMR3244, Sorbonne UniversitéInstitut de Génétique Humaine, CNRS et Université de Montpellier, Equipe labélisée Ligue contre le CancerInstitut de Génétique Humaine, CNRS et Université de Montpellier, Equipe labélisée Ligue contre le CancerWhile R-loops can alter cell homeostasis, it is unclear what determines their toxicity. Here, the authors, by using Top1 knockdown as a tool to enhance the formation of R-loops at certain genomic sites, reveal and characterize a proportion of R-loops that are more toxic to the cell by causing DNA damage.https://doi.org/10.1038/s41467-020-17858-2
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Alexy Promonet Ismaël Padioleau Yaqun Liu Lionel Sanz Anna Biernacka Anne-Lyne Schmitz Magdalena Skrzypczak Amélie Sarrazin Clément Mettling Maga Rowicka Krzysztof Ginalski Frédéric Chedin Chun-Long Chen Yea-Lih Lin Philippe Pasero
spellingShingle	Alexy Promonet Ismaël Padioleau Yaqun Liu Lionel Sanz Anna Biernacka Anne-Lyne Schmitz Magdalena Skrzypczak Amélie Sarrazin Clément Mettling Maga Rowicka Krzysztof Ginalski Frédéric Chedin Chun-Long Chen Yea-Lih Lin Philippe Pasero Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites Nature Communications
author_facet	Alexy Promonet Ismaël Padioleau Yaqun Liu Lionel Sanz Anna Biernacka Anne-Lyne Schmitz Magdalena Skrzypczak Amélie Sarrazin Clément Mettling Maga Rowicka Krzysztof Ginalski Frédéric Chedin Chun-Long Chen Yea-Lih Lin Philippe Pasero
author_sort	Alexy Promonet
title	Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites
title_short	Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites
title_full	Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites
title_fullStr	Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites
title_full_unstemmed	Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites
title_sort	topoisomerase 1 prevents replication stress at r-loop-enriched transcription termination sites
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-08-01
description	While R-loops can alter cell homeostasis, it is unclear what determines their toxicity. Here, the authors, by using Top1 knockdown as a tool to enhance the formation of R-loops at certain genomic sites, reveal and characterize a proportion of R-loops that are more toxic to the cell by causing DNA damage.
url	https://doi.org/10.1038/s41467-020-17858-2
work_keys_str_mv	AT alexypromonet topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT ismaelpadioleau topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT yaqunliu topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT lionelsanz topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT annabiernacka topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT annelyneschmitz topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT magdalenaskrzypczak topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT ameliesarrazin topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT clementmettling topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT magarowicka topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT krzysztofginalski topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT fredericchedin topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT chunlongchen topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT yealihlin topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites AT philippepasero topoisomerase1preventsreplicationstressatrloopenrichedtranscriptionterminationsites
_version_	1721215771317633024

Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

Similar Items