Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting
Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HR...
Main Authors: | , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2013-02-01
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Series: | Memórias do Instituto Oswaldo Cruz. |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100017&lng=en&tlng=en |
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by Joas Lucas da Silva, Gabriela Guimaraes Sousa Leite, Gisele Medeiros Bastos, Beatriz Cacciacarro Lucas, Daniel Keniti Shinohara, Joice Sayuri Takinami, Marcelo Miyata, Cristina Moreno Fajardo, André Ducati Luchessi, Clarice Queico Fujimura Leite, Rosilene Fressatti Cardoso, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata
Published 2013-02-01
Get full textPublished 2013-02-01
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