Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting

Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting

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Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HR...

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Bibliographic Details
Main Authors: Joas Lucas da Silva, Gabriela Guimaraes Sousa Leite, Gisele Medeiros Bastos, Beatriz Cacciacarro Lucas, Daniel Keniti Shinohara, Joice Sayuri Takinami, Marcelo Miyata, Cristina Moreno Fajardo, André Ducati Luchessi, Clarice Queico Fujimura Leite, Rosilene Fressatti Cardoso, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2013-02-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
drug resistance
rifampicin
Mycobacterium tuberculosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000100017&lng=en&tlng=en

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