Repeatable construction method for engineered zinc finger nuclease based on overlap extension PCR and TA-cloning.

Repeatable construction method for engineered zinc finger nuclease based on overlap extension PCR and TA-cloning.

Zinc finger nuclease (ZFN) is a useful tool for endogenous site-directed genome modification. The development of an easier, less expensive and repeatedly usable construction method for various sequences of ZFNs should contribute to the further widespread use of this technology. Here, we establish a...

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Bibliographic Details
Main Authors: Wataru Fujii, Kiyoshi Kano, Koji Sugiura, Kunihiko Naito
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3607563?pdf=render

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