Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells

Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells

Summary: The redox state of mitochondria is one indicator of the functional state of the organelles. Mitochondria are also the primary endogenous source of reactive oxygen species (ROS). Therefore, the redox state of the organelles also reflects their function in ROS production. Here, we provide ste...

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Main Authors: Pin-Chao Liao, Emily J. Yang, Liza A. Pon
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301477
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spelling doaj-bd022c02452b495cb1e39a0439d489a02020-12-21T04:48:56ZengElsevierSTAR Protocols2666-16672020-12-0113100160Live-Cell Imaging of Mitochondrial Redox State in Yeast CellsPin-Chao Liao0Emily J. Yang1Liza A. Pon2Department of Pathology and Cell Biology, Columbia University, New York, NY, USADepartment of Pathology and Cell Biology, Columbia University, New York, NY, USADepartment of Pathology and Cell Biology, Columbia University, New York, NY, USA; Corresponding authorSummary: The redox state of mitochondria is one indicator of the functional state of the organelles. Mitochondria are also the primary endogenous source of reactive oxygen species (ROS). Therefore, the redox state of the organelles also reflects their function in ROS production. Here, we provide step-by-step protocols for live-cell imaging and quantification of mitochondrial redox state using the genetically encoded fluorescent biosensor, mitochondria-targeted redox sensing GFP (mito-roGFP), and mitochondrial ROS using the membrane-permeant small molecule dihydroethidium (DHE) in budding yeast cells.For complete details on the use and execution of this protocol, please refer to Liao et al. (2020c).http://www.sciencedirect.com/science/article/pii/S2666166720301477
collection DOAJ
language English
format Article
sources DOAJ
author Pin-Chao Liao
Emily J. Yang
Liza A. Pon
spellingShingle Pin-Chao Liao
Emily J. Yang
Liza A. Pon
Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
STAR Protocols
author_facet Pin-Chao Liao
Emily J. Yang
Liza A. Pon
author_sort Pin-Chao Liao
title Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
title_short Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
title_full Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
title_fullStr Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
title_full_unstemmed Live-Cell Imaging of Mitochondrial Redox State in Yeast Cells
title_sort live-cell imaging of mitochondrial redox state in yeast cells
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: The redox state of mitochondria is one indicator of the functional state of the organelles. Mitochondria are also the primary endogenous source of reactive oxygen species (ROS). Therefore, the redox state of the organelles also reflects their function in ROS production. Here, we provide step-by-step protocols for live-cell imaging and quantification of mitochondrial redox state using the genetically encoded fluorescent biosensor, mitochondria-targeted redox sensing GFP (mito-roGFP), and mitochondrial ROS using the membrane-permeant small molecule dihydroethidium (DHE) in budding yeast cells.For complete details on the use and execution of this protocol, please refer to Liao et al. (2020c).
url http://www.sciencedirect.com/science/article/pii/S2666166720301477
work_keys_str_mv AT pinchaoliao livecellimagingofmitochondrialredoxstateinyeastcells
AT emilyjyang livecellimagingofmitochondrialredoxstateinyeastcells
AT lizaapon livecellimagingofmitochondrialredoxstateinyeastcells
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