Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles

Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles

Here we report on the immobilization of lipase (E.C 3.1.1.1) from Thermomyces lanuginosa on monodispersed Fe3O4 nanoparticles. The nanoparticles were prepared by a bottom-up chemical strategy based on low temperature thermal decomposition of an iron precursor in organic solvent in the presence of su...

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Main Authors: M. Sarno, L. Paciello, C. Cirillo, P. Parascandola, P. Ciambelli
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2016-05-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/3073
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spelling doaj-bcf73718814c45d9b49e4a06e0965faa2021-02-19T21:11:39ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162016-05-014910.3303/CET1649021Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic NanoparticlesM. SarnoL. PacielloC. CirilloP. ParascandolaP. CiambelliHere we report on the immobilization of lipase (E.C 3.1.1.1) from Thermomyces lanuginosa on monodispersed Fe3O4 nanoparticles. The nanoparticles were prepared by a bottom-up chemical strategy based on low temperature thermal decomposition of an iron precursor in organic solvent in the presence of surfactants. It offers many advantages, such as experimental easiness, potential low-cost fabrication, very high nanoparticles size control and reproducibility. The nanoparticles were treated with (3- aminopropyl) triethoxysilane (APTES) and then activated via the glutaraldehyde method to couple with lipase by a covalent bond. High recovered activity (77%) was obtained, a new washing procedure was developed to double the immobilization efficiency.https://www.cetjournal.it/index.php/cet/article/view/3073
collection DOAJ
language English
format Article
sources DOAJ
author M. Sarno
L. Paciello
C. Cirillo
P. Parascandola
P. Ciambelli
spellingShingle M. Sarno
L. Paciello
C. Cirillo
P. Parascandola
P. Ciambelli
Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
Chemical Engineering Transactions
author_facet M. Sarno
L. Paciello
C. Cirillo
P. Parascandola
P. Ciambelli
author_sort M. Sarno
title Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
title_short Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
title_full Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
title_fullStr Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
title_full_unstemmed Improvement of the Lipase Immobilization Procedure on Monodispersed Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles
title_sort improvement of the lipase immobilization procedure on monodispersed fe<sub>3</sub>o<sub>4</sub> magnetic nanoparticles
publisher AIDIC Servizi S.r.l.
series Chemical Engineering Transactions
issn 2283-9216
publishDate 2016-05-01
description Here we report on the immobilization of lipase (E.C 3.1.1.1) from Thermomyces lanuginosa on monodispersed Fe3O4 nanoparticles. The nanoparticles were prepared by a bottom-up chemical strategy based on low temperature thermal decomposition of an iron precursor in organic solvent in the presence of surfactants. It offers many advantages, such as experimental easiness, potential low-cost fabrication, very high nanoparticles size control and reproducibility. The nanoparticles were treated with (3- aminopropyl) triethoxysilane (APTES) and then activated via the glutaraldehyde method to couple with lipase by a covalent bond. High recovered activity (77%) was obtained, a new washing procedure was developed to double the immobilization efficiency.
url https://www.cetjournal.it/index.php/cet/article/view/3073
work_keys_str_mv AT msarno improvementofthelipaseimmobilizationprocedureonmonodispersedfesub3subosub4submagneticnanoparticles
AT lpaciello improvementofthelipaseimmobilizationprocedureonmonodispersedfesub3subosub4submagneticnanoparticles
AT ccirillo improvementofthelipaseimmobilizationprocedureonmonodispersedfesub3subosub4submagneticnanoparticles
AT pparascandola improvementofthelipaseimmobilizationprocedureonmonodispersedfesub3subosub4submagneticnanoparticles
AT pciambelli improvementofthelipaseimmobilizationprocedureonmonodispersedfesub3subosub4submagneticnanoparticles
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